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浙贝母基因组总DNA的初步分析与提取

[Preliminary analysis and isolation of genomic total DNA in Fritillaria thunbergii Miq].

作者信息

Gao W, Li Z, Xiao P, Fu R

机构信息

Institute of Medicinal Plant, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100094.

出版信息

Zhongguo Zhong Yao Za Zhi. 1998 Feb;23(2):79-81, 128.

Abstract

Genomic total DNA of leaf, fresh scale and dry scale in Fritillaria thumbergii were isolated by two different methods and analysed by agarose gel electrophoresis. The results show that the DNA contents are different under these two isolating methods. Two bands besides the band of genomic total DNA have been found by electrophoresis, these two bands deserve further study.

摘要

采用两种不同方法提取浙贝母叶片、鲜鳞片和干鳞片的基因组总DNA,并通过琼脂糖凝胶电泳进行分析。结果表明,两种提取方法下的DNA含量不同。电泳发现除基因组总DNA条带外还有两条条带,这两条条带值得进一步研究。

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