Hu P, Luo G A, Wang R J, Guo H Z, Li J S
Department of Chemistry, Tsinghua University, Beijing 100084.
Yao Xue Xue Bao. 1997 Jul;32(7):549-52.
A method for identification of semen Cuscutae, a traditional Chinese herb, was developed. The proteins in semen Cuscutae were extracted under acid or basic conditions and separated by high performance capillary electrophoresis. The electrophoretograms of acidic or basic extractants from Cuscuta chinensis Lam., Cuscuta australis R. Br. and Cuscuta japonica Choisy showed significant differences, which can be used to identify the three different semen Cuscutae. The results of the identification for 13 pharmacognosical samples agreed well with those of scanning electronic microscopy and tissue microanalysis.
建立了一种鉴定传统中药菟丝子种子的方法。在酸性或碱性条件下提取菟丝子种子中的蛋白质，并通过高效毛细管电泳进行分离。中国菟丝子、南方菟丝子和日本菟丝子的酸性或碱性提取物的电泳图谱显示出显著差异，可用于鉴别这三种不同的菟丝子种子。13个生药样品的鉴定结果与扫描电子显微镜和组织显微分析的结果吻合良好。
