Morin-Faure J, Marcollet M
Laboratoire d'Histologie-Embryologie-Cytogénétique, U.E.R. Médecine, Clermont-Ferrand/France.
Eur J Cell Biol. 1983 May;30(2):316-9.
The reaction of cis-dichlorodiamino platinum (cis-Pt) with the human metaphase chromosome was studied using both Giemsa staining and the action of anti-cis-Pt antibody. Two kinds of experiment were performed: (1) cis-Pt was placed in contact with cells in culture (in vivo tests), (2) cis-Pt was placed in contact with chromosomes fixed on slides (in vitro tests). In vivo, the presence of cis-Pt during phase G1 of the cell cycle is important for the reaction; in vitro, competition between cis-Pt and Giemsa for fixation on chromosomal DNA was shown to occur.
