Truchet S, Wietzerbin J, Debey P
INRA 806/MNHN EA 2703, IFR 63 Muséum National d'Histoire Naturelle, Institut de Biologie Physico-Chimique, 13, rue P. et M. Curie, 75005 Paris, France.
Mol Reprod Dev. 2001 Nov;60(3):319-30. doi: 10.1002/mrd.1094.
Cytokines and growth factors play important roles in implantation and maintenance of pregnancy, but also during early development. Among them interferon-gamma (IFNgamma) is highly expressed by mammalian trophoblast cells during implantation and seems to be involved in some cases of pregnancy loss. In the present study we investigated the possible presence of IFNgamma receptors (IFNGR) on mouse oocytes and preimplantation embryos. The two receptor chains IFNgammaRalpha (IFNGR-1) and IFNgammaRbeta (IFNGR-2) have been detected by indirect immunofluorescence at the surface of mouse oocytes (in germinal vesicle and metaphase II stages), as well as at all stages of in vitro embryo development from the one-cell to blastocyst stage. IFNGR appeared to colocalize partly with ganglioside GM1 at the cell surface of oocytes and embryos, indicating a possible preferential localization of this receptor in "rafts" microdomains. This was analyzed in more detail using software developed in the laboratory. IFNgamma was found to bind to its receptor at all stages analyzed. RT-PCR and Southern blot experiments confirmed the presence of the transcriptionally regulated IFNGR-2 chain mRNA, in mouse oocytes and preimplantation embryos. These results show, for the first time, that mouse oocytes and preimplantation embryos bear a complete and theoretically functional IFNGR, suggesting that this cytokine could play a role during early development.
细胞因子和生长因子在妊娠的着床和维持过程中发挥着重要作用，在早期发育阶段也是如此。其中，γ干扰素（IFNγ）在着床期间由哺乳动物滋养层细胞高度表达，并且在某些流产病例中似乎也有涉及。在本研究中，我们调查了小鼠卵母细胞和植入前胚胎上是否可能存在IFNγ受体（IFNGR）。通过间接免疫荧光在小鼠卵母细胞（生发泡期和中期II期）表面以及从单细胞到囊胚期的体外胚胎发育的所有阶段检测到了两条受体链，即IFNγRα（IFNGR-1）和IFNγRβ（IFNGR-2）。IFNGR似乎部分地与神经节苷脂GM1在卵母细胞和胚胎的细胞表面共定位，表明该受体可能优先定位于“筏”微结构域。使用实验室开发的软件对此进行了更详细的分析。发现在所有分析阶段IFNγ都能与其受体结合。RT-PCR和Southern印迹实验证实了在小鼠卵母细胞和植入前胚胎中转录调控的IFNGR-2链mRNA的存在。这些结果首次表明，小鼠卵母细胞和植入前胚胎带有完整且理论上具有功能的IFNGR，这表明这种细胞因子可能在早期发育中发挥作用。
