Engelhart D A, Jenkins A J
The Office of Cuyahoga County Coroner, Cleveland, Ohio 44106, USA.
J Anal Toxicol. 2001 Oct;25(7):612-5. doi: 10.1093/jat/25.7.612.
The disposable QED saliva alcohol test provides a very simple, fast, and reliable means for quantitative onsite alcohol detection. The purpose of this study was to determine if the QED test would be a useful tool for the determination of postmortem ethanol levels in cases where a rapid result was needed. QED results were compared with ethanol levels determined by headspace GC analysis. Both saliva and vitreous humor specimens were used for the evaluation. QED tests were initially attempted using the oral fluid from 50 individuals. Of these cases, 17 of the tests were valid with 8 positive results. For 23 cases the oral fluid was not attainable, and for 10 cases, the sample was contaminated with blood making the tests invalid. The correlation between the oral fluid results and the blood headspace GC analysis was poor (r = 0.8345) over the range of 0.01-0.29 g/dL. Vitreous specimens were found to be the matrix of choice for analyzing postmortem cases using the QED. Only 6 of 171 specimens were found to be unsuitable. The QED results correlated well with the headspace GC analysis (r = 0.9931, n = 165). When using ethanol levels > 0.02 g/dL (n = 126), an average vitreous (GC)/blood ratio of 1.16 correlated well with the average QED/blood ratio of 1.22. Although the QED saliva alcohol test does not appear to be useful in determining postmortem saliva ethanol levels, it does provide accurate results when using postmortem vitreous humor as the testing matrix.
一次性QED唾液酒精检测提供了一种非常简单、快速且可靠的现场定量酒精检测方法。本研究的目的是确定在需要快速结果的情况下，QED检测是否会成为测定死后乙醇水平的有用工具。将QED检测结果与通过顶空气相色谱分析测定的乙醇水平进行比较。同时使用唾液和玻璃体液样本进行评估。最初尝试对50个人的口腔液进行QED检测。在这些案例中，17次检测有效，其中8次结果为阳性。23个案例无法获取口腔液，10个案例的样本被血液污染，导致检测无效。在0.01 - 0.29 g/dL范围内，口腔液检测结果与血液顶空气相色谱分析的相关性较差（r = 0.8345）。发现玻璃体液样本是使用QED分析死后案例的首选基质。在171个样本中，仅发现6个不适合。QED检测结果与顶空气相色谱分析相关性良好（r = 0.9931，n = 165）。当使用乙醇水平> 0.02 g/dL（n = 126）时，玻璃体液（气相色谱法）/血液的平均比值为1.16，与QED/血液的平均比值1.22相关性良好。尽管QED唾液酒精检测似乎在测定死后唾液乙醇水平方面并无用处，但在将死后玻璃体液作为检测基质时，它确实能提供准确的结果。
