Cantos E, Espín J C, Tomás-Barberán F A
Laboratorio de Fitoquímica, Departamento Ciencia y Tecnología de Alimentos, CEBAS-CSIC, P. O. Box 4195, 30080 Murcia, Spain.
J Agric Food Chem. 2001 Oct;49(10):5052-8. doi: 10.1021/jf010366a.
A modeling method for the induction of resveratrol synthesis by UV irradiation pulses in Napoleon table grapes is proposed. The method is based on the combination of four main parameters: irradiation power (IW), irradiation time (IT), irradiation distance (ID), and number of elapsed days to achieve the highest resveratrol accumulation (D(m)). Maximum resveratrol content (11-fold higher than untreated grapes) was achieved using the combination: IW = 510 W, IT = 30 s, ID = 40 cm, and D(m) = 3 days. Sensory characteristics and main features of irradiated grapes (color, weight, firmness, flavor, size, ripening index and vitamin C content) remained unaltered after 1 week of storage. UV induction signal migrated to the hidden side of the grape skin with a delay of 3 days as compared to the directly irradiated side. Phenolic compounds were not detected in Napoleon grape flesh. Resveratrol content per standard serving (200 g) of irradiated grape was about 3 mg, an amount more than 10-fold higher than that of untreated Napoleon grapes. This means that a serving of irradiated grape (unpeeled) could supply the resveratrol content equivalent to 3 glasses of a red wine with high resveratrol content ( approximately 1 mg/glass). Therefore, controlled UV irradiation pulses are useful as a simple postharvest treatment (and alternative to genetic engineering) to obtain possible "functional" grapes (with enhanced health-promoting properties) as a dietary source of high resveratrol content.
提出了一种通过紫外线照射脉冲诱导拿破仑鲜食葡萄中白藜芦醇合成的建模方法。该方法基于四个主要参数的组合：照射功率（IW）、照射时间（IT）、照射距离（ID）以及达到最高白藜芦醇积累量所经过的天数（D(m)）。通过IW = 510 W、IT = 30 s、ID = 40 cm和D(m) = 3天的组合，实现了最大白藜芦醇含量（比未处理的葡萄高11倍）。辐照葡萄的感官特性和主要特征（颜色、重量、硬度、风味、大小、成熟指数和维生素C含量）在储存1周后保持不变。与直接照射的一侧相比，紫外线诱导信号延迟3天迁移到葡萄皮的背侧。在拿破仑葡萄果肉中未检测到酚类化合物。每标准份（200克）辐照葡萄中的白藜芦醇含量约为mg，这一含量比未处理的拿破仑葡萄高10倍以上。这意味着一份辐照葡萄（带皮）所提供的白藜芦醇含量相当于3杯白藜芦醇含量高的红酒（约1毫克/杯）。因此，可控的紫外线照射脉冲作为一种简单的采后处理方法（可替代基因工程）是有用的，能够获得可能具有“功能性”的葡萄（具有增强的促进健康特性），作为高白藜芦醇含量的饮食来源。
