Saruhan-Direskeneli G, Celet B, Eksioglu-Demiralp E, Direskeneli H
Department of Physiology, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey.
Immunol Lett. 2001 Dec 3;79(3):203-8. doi: 10.1016/s0165-2478(01)00280-2.
Heat shock protein (HSP, 60/65 kDa) is investigated as a candidate autoantigen in Behçet's disease (BD), a systemic vasculitis of unknown origin, and a prominent response to 'disease-specific epitopes' of mycobacterial and human HSP60/65 is described in BD patients. In this study, long-term T cell lines from peripheral blood of BD patients (n=6) and controls (n=7) were stimulated with mycobacterial recombinant HSP and purified protein derivate (PPD) and expanded with IL-2. In the BD group, 15 out 27 and in the controls, 25 out of 35 PPD specific T cell lines have responded to the synthetic peptides of the human HSP60. Out of the primarily HSP-specific T cell lines, 17/23 in patients and 8/8 in controls did recognize a peptide of human origin. T cell lines specifically reactive to 136-150, 179-197, 244-258 and 336-351 could be raised with similar frequency in both groups. In contrast to a previous report, T cells also reacted to peptide 425-441 frequently in both groups. The results demonstrate that the human proliferative response to mycobacterial HSP may also target the self-protein in both BD patients and controls. However, the responsive T cells may have different effects depending on their functional features such as cytokine secretions.
热休克蛋白（HSP，60/65 kDa）被作为白塞病（BD）的候选自身抗原进行研究，白塞病是一种病因不明的系统性血管炎，BD患者对分枝杆菌和人HSP60/65的“疾病特异性表位”有显著反应。在本研究中，用分枝杆菌重组HSP和纯化蛋白衍生物（PPD）刺激BD患者（n = 6）和对照（n = 7）外周血的长期T细胞系，并用IL-2进行扩增。在BD组中，27个PPD特异性T细胞系中有15个，在对照组中，35个中有25个对人HSP60的合成肽有反应。在最初的HSP特异性T细胞系中，患者组23个中有17个，对照组8个中有8个识别出人类来源的肽段。两组中对136 - 150、179 - 197、244 - 258和336 - 351特异性反应的T细胞系出现频率相似。与之前的一份报告不同，两组中的T细胞对肽段425 - 441也常有反应。结果表明，BD患者和对照组中对分枝杆菌HSP的人类增殖反应也可能针对自身蛋白。然而，反应性T细胞可能因其细胞因子分泌等功能特性而产生不同影响。
