[Analysis of the protein of Haemophilus influenzae].

OBJECTIVE

To understand Haemophilus influenzae special protein and to help for preparing Haemophilus influenzae Vaccine.

METHODS

Proteins from isolates of Haemophilus influenzae were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

RESULTS

23 isolates were from nasopharyngeal secretions of children with pneumonia and 33 from pharyx swab of healthy children. Conventional tests showed that 79% of fifty-six strains of Haemophilus influenzae were non-serotypable and all belonged to one of eight biotypes. The common biotypes were II (48%). All strains contained more than 10 proteins with molecular weights of 96,000, 86,000, 55,000, 51,000, 46,000, 40,000, 39,000, 31,000, 29,000, 25,000, and 16,000. Among them, three were major proteins (31,000, 51,000 and 96,000) and different proteins were mainly between peptide 31,000 and 51,000. A possible association between biotype and protein was unclear.

CONCLUSION

Analysis of protein of Haemophilus influenzae is helpful for epidemiological study of non-serotypable Haemophilus influenzae and making Haemophilus influenzae vaccine.