Xie J, Xue B, Wang L
Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020.
Zhonghua Yi Xue Za Zhi. 1999 Jan;79(1):61-3.
To understand Haemophilus influenzae special protein and to help for preparing Haemophilus influenzae Vaccine.
Proteins from isolates of Haemophilus influenzae were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
23 isolates were from nasopharyngeal secretions of children with pneumonia and 33 from pharyx swab of healthy children. Conventional tests showed that 79% of fifty-six strains of Haemophilus influenzae were non-serotypable and all belonged to one of eight biotypes. The common biotypes were II (48%). All strains contained more than 10 proteins with molecular weights of 96,000, 86,000, 55,000, 51,000, 46,000, 40,000, 39,000, 31,000, 29,000, 25,000, and 16,000. Among them, three were major proteins (31,000, 51,000 and 96,000) and different proteins were mainly between peptide 31,000 and 51,000. A possible association between biotype and protein was unclear.
Analysis of protein of Haemophilus influenzae is helpful for epidemiological study of non-serotypable Haemophilus influenzae and making Haemophilus influenzae vaccine.
了解流感嗜血杆菌特殊蛋白，以辅助制备流感嗜血杆菌疫苗。
采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）对流感嗜血杆菌分离株的蛋白质进行检测。
23株分离株来自肺炎患儿的鼻咽分泌物，33株来自健康儿童的咽拭子。常规检测显示，56株流感嗜血杆菌中有79%不可分型，均属于8种生物型之一。常见生物型为II型（48%）。所有菌株均含有10种以上分子量分别为96,000、86,000、55,000、51,000、46,000、40,000、39,000、31,000、29,000、25,000和16,000的蛋白质。其中，三种为主要蛋白（31,000、51,000和96,000），不同蛋白主要存在于31,000和51,000肽段之间。生物型与蛋白之间的可能关联尚不清楚。
流感嗜血杆菌蛋白分析有助于不可分型流感嗜血杆菌的流行病学研究及流感嗜血杆菌疫苗的制备。
