[CD28共刺激淋巴细胞中免疫激活基因的表达及其作为肾移植诊断工具的应用]
[Immune activation genes expression in CD28-costimulated lymphocytes and its application as a diagnostic tool in kidney transplantation].
作者信息
Zhou P, Wang X, Tang X
机构信息
Center of Renal Transplantation, Shanghai First People's Hospital, Shanghai 200080.
出版信息
Zhonghua Yi Xue Za Zhi. 1999 Apr;79(4):257-9.
OBJECTIVE
To investigate the detection of immune activation genes expression as an indicator for the diagnosis of immunological rejection in renal transplantation.
METHODS
In 61 renal allograft recipients, the immune activation genes expression of CD28-costimulated peripheral blood mononuclear cells (PBMC) was studied by RT-PCR.
RESULTS
The mRNA expression positive rate of IL-10, CD40L, Fas, Perforin, IL-2, IFN-gamma, FasL, bcl-xL, Granzyme B (GB), TGF-beta 1 and IL-4 was 91.8%, 70.5%, 88.5%, 96.7%, 41%, 54.1%, 60.7%, 67.2%, 67.2%, 54.1% and 32.8% respectively. IL-2, IFN-gamma, FasL and Granzyme B were screened out as indicators for the diagnosis of allograft rejection. Any three or more of IL-2, IFN-gamma, FasL, and Granzyme B genes expression could be used as a diagnostic tool for rejection with much favorable sensitivity (93%) and specificity (75%).
CONCLUSION
The detection of IL-2, IFN-gamma, FasL and Granzyme B genes expression is beneficial for the evaluation of immunological state in renal allograft recipients. This non-invasive method can be used for clinical diagnosis of allograft rejection.
目的
研究免疫激活基因表达检测作为肾移植免疫排斥诊断指标的可行性。
方法
采用逆转录聚合酶链反应（RT-PCR）技术，检测61例肾移植受者CD28共刺激外周血单个核细胞（PBMC）免疫激活基因的表达。
结果
IL-10、CD40L、Fas、穿孔素、IL-2、IFN-γ、FasL、bcl-xL、颗粒酶B（GB）、TGF-β1和IL-4的mRNA表达阳性率分别为91.8%、70.5%、88.5%、96.7%、41%、54.1%、60.7%、67.2%、67.2%、54.1%和32.8%。筛选出IL-2、IFN-γ、FasL和颗粒酶B作为诊断移植排斥反应的指标。IL-2、IFN-γ、FasL和颗粒酶B中任意三个或更多基因表达可作为排斥反应的诊断工具，具有良好的敏感性（93%）和特异性（75%）。
结论
检测IL-2、IFN-γ、FasL和颗粒酶B基因表达有助于评估肾移植受者的免疫状态。这种非侵入性方法可用于临床诊断移植排斥反应。
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