[The effects of 6A8 cDNA transfection on biological behavior of tumor cells].

OBJECTIVE

To investigate the effects of antisense 6A8 cDNA on the malignant behavior of tumor.

METHODS

Highly metastatic clonal variant CNE-2L2 from nasopharygeal carcinoma cell line(CNE-2Z) was transfected with antisense 6A8 cDNA or mock plasmid. Their adhesion, locomotion, proteolytic ability, in vitro invasion, in vivo growth and metastasis were comparatively studied with the wild CNE-2L2 cells.

RESULTS

Antisense 6A8 cDNA transfected CNE-2L2 showed less adhesion to FN and LN, weakened locomotive ability and proteolytic ability, retarded in vivo growth and much less metastasis.

CONCLUSION

Transfection of antisense 6A8 cDNA obviously decreased the malignant behavior of tumor cells.