儿童B系急性淋巴细胞白血病中TEL-AML1融合基因的研究
[Study of TEL-AML1 fusion gene in childhood B-lineage acute lymphoblastic leukemia].
作者信息
Niu C, Huang W, Su X
机构信息
Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025.
出版信息
Zhonghua Xue Ye Xue Za Zhi. 1999 Feb;20(2):61-4.
OBJECTIVE
To determine the incidence of TEL-AML1 fusion gene and its role in clinical diagnosis and prognosis of childhood B-lineage acute lymphoblastic leukemia (B-ALL), and to compare the two techniques: nested RT-PCR and dual-color fluorescence in situ hybridization (FISH).
METHODS
Nested RT-PCR and dual-color FISH techniques were used to detect TEL-AML1 fusion gene.
RESULTS AND CONCLUSION
TEL-AML1 fusion transcript was found in 22.5%(9/40) of patients with B-ALL by RT-PCR. The prognosis of these TEL-AML1 positive patients was relatively good and the complete remission rate was 100%. Both RT-PCR and FISH techniques were proved to be useful for detecting TEL-AML1 fusion gene and suitable for clinical diagnosis and prognosis evaluation.
目的
确定TEL-AML1融合基因在儿童B系急性淋巴细胞白血病（B-ALL）临床诊断及预后中的发生率及其作用，并比较巢式逆转录聚合酶链反应（RT-PCR）和双色荧光原位杂交（FISH）这两种技术。
方法
采用巢式RT-PCR和双色FISH技术检测TEL-AML1融合基因。
结果与结论
通过RT-PCR在22.5%（9/40）的B-ALL患者中发现了TEL-AML1融合转录本。这些TEL-AML1阳性患者预后相对较好，完全缓解率为100%。RT-PCR和FISH技术均被证明可用于检测TEL-AML1融合基因，适用于临床诊断及预后评估。
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