Yao B, Guo M, Pian H
Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100039.
Zhonghua Xue Ye Xue Za Zhi. 1999 Mar;20(3):137-9.
To define the relationship between Ph chromosome and BCR/ABL mRNA expression in chronic myeloid leukemia (CML) patients, and compare cytogenetic analysis and PCR method.
Marrow or peripheral blood samples from 33 CML patients were analyzed by R banding technique and PCR method. Eleven patients who received allogeneic BMT or alpha-IFN + low dose HU were followed up.
In 33 patients, 29 were Ph(+), 30 expressed BCR/ABL mRNA. In 6 patients who received BMT, 4 were Ph(-), 2 were Ph(+). However, 2 patients were (-), 4 were (+) by PCR method. In 5 patients who received alpha-IFN, the percentage of Ph(+) cells reduced after 6 and 12 months treatment than before treatment in 2 cases respectively. In 41 samples, 34 cases were consistent by 2 methods.
Each method, Cytogenetic analysis or PCR has its advantage and defect. The better method are integration of 2 methods in diagnosis and treatment monitoring of CML patients, so that provide real and beneficial information for clinic.
明确慢性髓性白血病（CML）患者中Ph染色体与BCR/ABL mRNA表达之间的关系，并比较细胞遗传学分析和聚合酶链反应（PCR）方法。
采用R显带技术和PCR方法对33例CML患者的骨髓或外周血样本进行分析。对11例接受异基因骨髓移植（BMT）或α-干扰素 + 小剂量羟基脲（HU）治疗的患者进行随访。
33例患者中，29例为Ph（+），30例表达BCR/ABL mRNA。在6例接受BMT的患者中，4例为Ph（-），2例为Ph（+）。然而，通过PCR方法检测，2例为（-），4例为（+）。在5例接受α-干扰素治疗的患者中，分别有2例在治疗6个月和12个月后Ph（+）细胞百分比较治疗前降低。在41份样本中，两种方法检测结果一致的有34例。
细胞遗传学分析和PCR这两种方法各有优缺点。在CML患者的诊断和治疗监测中，较好的方法是将两种方法结合，以便为临床提供真实且有益的信息。
