烧伤愈合液对角质形成细胞和成纤维细胞增殖及胶原格子收缩的影响。
Effect of burn healing liquid on keratinocyte and fibroblast proliferation and on collagen lattice contraction.
作者信息
Su Y, Chen B, Thang P T, Hughes M A, Xu X, Cherry G W
机构信息
Department of Burn Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
出版信息
Chin Med J (Engl). 1999 Aug;112(8):720-4.
OBJECTIVES
To investigate the effect of Burn Healing Liquid (BHL) on the proliferation of keratinocytes and fibroblasts and to explore the potential effect of BHL on fibroblast contraction.
METHODS
Human keratinocytes and dermal fibroblasts were cultured in media containing serial dilutions of BHL followed by cell proliferation determination assessed with MTT (3-[4, 5-dimehtylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide) assay at different time intervals. The in vitro collagen lattice contraction model was utilized for determining the contractility of fibroblasts cultured in BHL containing medium.
RESULTS
The 1:10(7) dilution of BHL enhanced the growth of both keratinocytes and fibroblasts whereas the 1:10 dilution increased the growth of keratinocytes only. Collagen lattice contraction was inhibited dose-dependently by BHL and such an inhibition could be reversed by switching BHL containing medium to normal medium containing 10% fetal calf serum.
CONCLUSION
BHL enhances the growth of both keratinocytes and fibroblasts and reversibly inhibits the fibroblast contraction in collagen lattice.
目的
研究烧伤愈合液（BHL）对角质形成细胞和成纤维细胞增殖的影响，并探讨BHL对成纤维细胞收缩的潜在作用。
方法
将人角质形成细胞和真皮成纤维细胞培养于含有系列稀释度BHL的培养基中，随后在不同时间间隔用MTT（3-[4,5-二甲基噻唑-2-基]-2,5-二苯基溴化四氮唑）法评估细胞增殖情况。采用体外胶原格子收缩模型测定在含BHL培养基中培养的成纤维细胞的收缩能力。
结果
BHL 1:10(7)稀释液可促进角质形成细胞和成纤维细胞的生长，而1:10稀释液仅增加角质形成细胞的生长。BHL对胶原格子收缩具有剂量依赖性抑制作用，将含BHL的培养基换成含10%胎牛血清的正常培养基可逆转这种抑制作用。
结论
BHL可促进角质形成细胞和成纤维细胞的生长，并可逆性抑制胶原格子中成纤维细胞的收缩。
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