大豆基婴儿配方奶粉中维生素B6的液相色谱分析。
Liquid chromatographic analysis of vitamin B6 in soy-based infant formula.
作者信息
Mann D L, Chase G W, Eitenmiller R R
机构信息
U.S. Food and Drug Administration, Southeast Regional Laboratory, Atlanta, GA 30309, USA.
出版信息
J AOAC Int. 2001 Sep-Oct;84(5):1593-9.
A liquid chromatographic (LC) method is described for determination of total vitamin B6 in soy-based infant formula. Total vitamin B6 is quantitated by using ion-pair LC after precolumn transformation of phosphorylated and free vitamers into pyridoxol. The limit of detection is 0.3 ng and the limit of quantitation is 1.0 ng on-column (injection volume = 100 microL). Linear response ranged from 39 to 616 ng/mL (r2 = 0.99986). Analysis of a soy-based infant formula control fortified at 6 different concentration levels gave recoveries that averaged 104%. Assay of SRM 1846 gave results within the certified range (8.6 +/- 0.086 mg/kg versus the certified value of 8.4 +/- 1.0 mg/kg). The method provides a rapid and specific assay for the analysis of total vitamin B6 in fortified soy-based infant formula.
本文描述了一种用于测定大豆基婴儿配方奶粉中总维生素B6的液相色谱（LC）方法。在将磷酸化和游离维生素原预柱转化为吡哆醇后，采用离子对LC对总维生素B6进行定量。检测限为0.3 ng，柱上定量限为1.0 ng（进样体积 = 100 μL）。线性响应范围为39至616 ng/mL（r2 = 0.99986）。对添加了6种不同浓度水平的大豆基婴儿配方奶粉对照品进行分析，平均回收率为104%。对标准参考物质1846的测定结果在认证范围内（8.6 +/- 0.086 mg/kg，而认证值为8.4 +/- 1.0 mg/kg）。该方法为强化大豆基婴儿配方奶粉中总维生素B6的分析提供了一种快速且特异的检测方法。
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