Hans M A, Heinzle E, Wittmann C
Biochemical Engineering Institute, Saarland University, Germany.
Appl Microbiol Biotechnol. 2001 Sep;56(5-6):776-9. doi: 10.1007/s002530100708.
The dynamics of intracellular amino acid pools were determined in batch cultures of Saccharomyces cerevisiae. Immediate termination of metabolic activity was found to be necessary for accurate quantification of in vivo concentrations of intracellular amino acids, due to significant changes in most intracellular amino acid pools observed during extraction without an instantaneous stop of the metabolism. The method applied to batch-cultures of S. cerevisiae on glucose revealed complex dynamics in intracellular amino acid pools. The most drastic changes were observed during the diauxic shift and at the entry into the stationary phase. Even during phases of exponential growth on glucose and ethanol, cells showed significant variations in intracellular amino acid concentrations. The method presented can be used to investigate the physiology of yeast cultures, including industrially relevant batch and fed-batch processes.
在酿酒酵母的分批培养中测定了细胞内氨基酸库的动态变化。由于在提取过程中未瞬间停止代谢时观察到大多数细胞内氨基酸库有显著变化，因此发现有必要立即终止代谢活性，以便准确量化细胞内氨基酸的体内浓度。应用于以葡萄糖为培养基的酿酒酵母分批培养的方法揭示了细胞内氨基酸库的复杂动态变化。在双相转变期间和进入稳定期时观察到最剧烈的变化。即使在以葡萄糖和乙醇为碳源的指数生长阶段，细胞内氨基酸浓度也有显著变化。所提出的方法可用于研究酵母培养物的生理学，包括与工业相关的分批和补料分批过程。
