秀丽隐杆线虫的slit蛋白通过SAX-3/Robo受体在中线、背腹和前后轴导向中发挥作用。

C. elegans slit acts in midline, dorsal-ventral, and anterior-posterior guidance via the SAX-3/Robo receptor.

作者信息

Hao J C, Yu T W, Fujisawa K, Culotti J G, Gengyo-Ando K, Mitani S, Moulder G, Barstead R, Tessier-Lavigne M, Bargmann C I

机构信息

Howard Hughes Medical Institute, Department of Anatomy, The University of California, San Francisco, CA 94143, USA.

出版信息

Neuron. 2001 Oct 11;32(1):25-38. doi: 10.1016/s0896-6273(01)00448-2.

DOI:10.1016/s0896-6273(01)00448-2

PMID:11604136

Abstract

Robo receptors interact with ligands of the Slit family. The nematode C. elegans has one Robo receptor (SAX-3) and one Slit protein (SLT-1), which direct ventral axon guidance and guidance at the midline. In larvae, slt-1 expression in dorsal muscles repels axons to promote ventral guidance. SLT-1 acts through the SAX-3 receptor, in parallel with the ventral attractant UNC-6 (Netrin). Removing both UNC-6 and SLT-1 eliminates all ventral guidance information for some axons, revealing an underlying longitudinal guidance pathway. In the embryo, slt-1 is expressed at high levels in anterior epidermis. Embryonic expression of SLT-1 provides anterior-posterior guidance information to migrating CAN neurons. Surprisingly, slt-1 mutants do not exhibit the nerve ring and epithelial defects of sax-3 mutants, suggesting that SAX-3 has both Slit-dependent and Slit-independent functions in development.

摘要

Robo受体与Slit家族的配体相互作用。线虫秀丽隐杆线虫有一个Robo受体（SAX-3）和一个Slit蛋白（SLT-1），它们指导腹侧轴突导向和中线处的导向。在幼虫中，背侧肌肉中slt-1的表达排斥轴突以促进腹侧导向。SLT-1通过SAX-3受体起作用，与腹侧吸引剂UNC-6（Netrin）协同作用。去除UNC-6和SLT-1会消除某些轴突的所有腹侧导向信息，揭示了一条潜在的纵向导向途径。在胚胎中，slt-1在前表皮中高水平表达。SLT-1的胚胎表达为迁移的CAN神经元提供前后导向信息。令人惊讶的是，slt-1突变体没有表现出sax-3突变体的神经环和上皮缺陷，这表明SAX-3在发育过程中具有依赖Slit和不依赖Slit的功能。