Roberson M, Neri A, Oppenheimer S B
Science. 1975 Aug 22;189(4203):639-40. doi: 10.1126/science.1162345.
The early 32- to 64-cell stage of the sea urchin embryo consists of three cell types, easily distinguishable by size: micromeres, mesomeres, and macromeres. Only the micromeres are migratory. Treatment of dissociated sea urchin embryo cells with fluorescein-labeled concanavalin A (Con A) revealed a Con A-induced highly clustered or capped distribution of receptor sites on the micromeres. Concanavalin A did not induce significant clustering or capping of receptor sites on the mesomeres or macromeres. The results indicate that Con A receptor sites are more mobile on specific population of malignant-like migratory embryonic cells.
海胆胚胎的早期32至64细胞阶段由三种细胞类型组成，可根据大小轻松区分：小分裂球、中分裂球和大分裂球。只有小分裂球具有迁移性。用荧光素标记的伴刀豆球蛋白A（Con A）处理解离的海胆胚胎细胞，结果显示在小分裂球上Con A诱导受体位点呈现高度聚集或帽状分布。伴刀豆球蛋白A并未在中分裂球或大分裂球上诱导受体位点出现显著的聚集或帽状分布。结果表明，Con A受体位点在特定的类恶性迁移胚胎细胞群体上更具流动性。
