大鼠空肠的表面活性剂与生物电特性

Surfactants and bioelectric properties of rat jejunum.

作者信息

Feldman D S, Rabinovitch S, Feldman E B

出版信息

Am J Dig Dis. 1975 Sep;20(9):866-70. doi: 10.1007/BF01070956.

Abstract

The effects of surfactants on bioelectric properties of rat jejunum were determined. Tween 80 (nonionic) and sodium dodecylsulfate (anionic) increased transmural potential differences 20-34% over values in Krebs-Ringer bicarbonate buffer. Short-circuit currents increased 66-112% and net tissue resistance decreased 19-30%. The cationic surfactant cetrimide decreased transmural potential 23%; short-circuit current decreased 32%, and resistance increased 22%. When sulfate replaced chloride in buffer, surfactant effects were minimized or reversed suggesting a role of Cl- flux in the bioelectric effects. Cationic surfactant effects on current and resistance were in the same direction as, but of greater and lesser magnitude, respectively, than alterations observed with bile salts. The current increase was of greater magnitude and resistance decrease less marked. Surfactant molecules may interact specifically with membranes in a more complex manner than simple "detergency".

摘要

测定了表面活性剂对大鼠空肠生物电特性的影响。吐温80（非离子型）和十二烷基硫酸钠（阴离子型）使跨壁电位差比在 Krebs-Ringer 碳酸氢盐缓冲液中的值增加了20 - 34%。短路电流增加了66 - 112%，组织净电阻降低了19 - 30%。阳离子表面活性剂西曲溴铵使跨壁电位降低了23%；短路电流降低了32%，电阻增加了22%。当缓冲液中的硫酸盐取代氯化物时，表面活性剂的作用最小化或逆转，这表明氯离子通量在生物电效应中起作用。阳离子表面活性剂对电流和电阻的影响方向与胆盐观察到的变化相同，但幅度分别更大和更小。电流增加幅度更大，电阻降低不明显。表面活性剂分子可能以比简单“去污作用”更复杂的方式与膜发生特异性相互作用。