Iarovaia O, Razin S V, Linares-Cruz G, Sjakste N, Scherrer K
Institut Jacques Monod, Université Paris 7, 2 Place Jussieu, Tour 43, Paris cedex 05, 75251, France.
Exp Cell Res. 2001 Nov 1;270(2):159-65. doi: 10.1006/excr.2001.5332.
Using hybridization in situ with a ribo-probe recognizing transcripts of the chicken alpha A globin gene, we show here that in proliferating AEV-transformed erythroblasts this gene is strongly transcribed, but the corresponding transcripts are retained in the nuclei. Most surprisingly, this globin RNA accumulates in the perinucleolar areas in a pattern never observed before. Upon induction of cells to differentiate, leading to productive expression of the hemoglobins, the transcripts of the alpha A globin gene were found for the most part in the cytoplasm. In the nuclei of differentiated cells, the globin RNA is concentrated in one or two specific spots, which are likely to represent the "processing centers" (PCs) of the globin RNA. The results presented indicate that posttranscriptional steps of regulation involving in particular the perinuclear areas are of major importance for erythroid differentiation.
利用与识别鸡αA珠蛋白基因转录本的核糖核酸探针进行原位杂交，我们在此表明，在增殖的AEV转化红细胞中，该基因强烈转录，但相应的转录本保留在细胞核中。最令人惊讶的是，这种珠蛋白RNA以一种前所未有的模式聚集在核仁周围区域。当诱导细胞分化，导致血红蛋白的有效表达时，αA珠蛋白基因的转录本大部分存在于细胞质中。在分化细胞的细胞核中，珠蛋白RNA集中在一个或两个特定的斑点中，这些斑点可能代表珠蛋白RNA的“加工中心”（PCs）。所呈现的结果表明，特别是涉及核周区域的转录后调控步骤对红细胞分化至关重要。
