Kolobanova S V, Filippova I Iu, Lysogorskaia E N, Bacheva A V, Oksenoĭt E S, Stepanov V M
Moscow State University, Chemical Faculty, Vorob'evy gory, Moscow, 119899 Russia.
Bioorg Khim. 2001 Sep-Oct;27(5):347-51. doi: 10.1023/a:1012388229945.
The segment condensation of peptides on a solid phase (Aminosilochrom) in organic medium catalyzed by a subtilisin complex with sodium dodecylsulfate was studied. The dependence of the efficiency of the enzymatic coupling of tripeptides with the basic structure X-Ala-Ala-Y-OMe [where X = Z, Boc, or Dnp and Y = Leu or Glu(OMe)] on the spacer content on the support and on the structure of the acylating component was investigated. The tripeptide segments were successively coupled to Aminosilochrom containing the Met-Ala-Gly spacer, and the peptidylaminosilochroms Dnp-Ala-Ala-Leu-Ala-Ala-Leu-Ala-Ala-Glu(OMe)-Met-Ala-Gly-A and Dnp-Ala-Ala-Leu-Ala-Ala-Glu(OMe)-Ala-Ala-Leu-Met-Ala-Gly-A (A is the Aminosilochrom residue) were obtained in satisfactory yields. It was shown by these examples that the second and third segments are attached in yields higher than that for the first segment and the coupling efficiency does not depend on the amino acid composition of the acylating component.
研究了在有机介质中，由枯草杆菌蛋白酶与十二烷基硫酸钠形成的复合物催化的肽在固相（氨基硅色原）上的片段缩合反应。研究了具有基本结构X-Ala-Ala-Y-OMe [其中X = Z、Boc或Dnp，Y = Leu或Glu(OMe)]的三肽的酶促偶联效率对载体上间隔基团含量以及酰化组分结构的依赖性。将三肽片段依次偶联到含有Met-Ala-Gly间隔基团的氨基硅色原上，得到了产率令人满意的肽基氨基硅色原Dnp-Ala-Ala-Leu-Ala-Ala-Leu-Ala-Ala-Glu(OMe)-Met-Ala-Gly-A和Dnp-Ala-Ala-Leu-Ala-Ala-Glu(OMe)-Ala-Ala-Leu-Met-Ala-Gly-A（A为氨基硅色原残基）。这些例子表明，第二段和第三段的连接产率高于第一段，且偶联效率不取决于酰化组分的氨基酸组成。
