Tomilova N B, Tomilov A A, Ogarkova O A, Soldatova O P, Tarasov V A
Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991 Russia.
Genetika. 2001 Sep;37(9):1251-7.
We present data on the phenotype identification and genetic analysis of offspring in three lines of dominant morphological mutants of Arabidopsis thaliana having drastically reduced fertility (a sterile calluslike mutant, a flower mutant, and a dwarf mutant) and in five lines of recessive morphological mutants (four mutants with lethal seedlings and one pigmentation mutant). The mutants were selected from a collection of transgenic plants that had genomes carrying a T-DNA insertion of plasmid vectors pLD3 and pPCVRN4; the collection was created earlier via agrobacterial transformation of germinating seeds. The results presented here were obtained using compensation of hormonal imbalance in the insertional morphological mutants of A. thaliana by exogenous hormones.
我们展示了关于拟南芥三个显性形态突变体系(育性大幅降低的不育愈伤组织样突变体、花突变体和矮化突变体)以及五个隐性形态突变体系(四个致死幼苗突变体和一个色素沉着突变体)后代的表型鉴定和遗传分析数据。这些突变体是从携带质粒载体pLD3和pPCVRN4的T-DNA插入基因组的转基因植物集合中筛选出来的;该集合是通过农杆菌转化萌发种子在早期创建的。此处展示的结果是利用外源激素补偿拟南芥插入形态突变体中的激素失衡而获得的。