Rubin H, Trelease R N
J Parasitol. 1975 Aug;61(4):577-88.
Triglyceride utilization is correlated with glycogen resynthesis in developing Ascaris suum larvae. Glycogen content, determined by amyloglucosidase hydrolysis at 2-day intervals, decreases sharply during embryonation from 15% (per mg dry weight) at day 4 to 2% at day 12. Thereafter glycogen increases 3-fold through day 20, marking the resynthesis period. Triglyceride lipid droplets are confirmed primarily to the posterior half of the larvae and mark the site of the interconversion. Although they serve as precursors to glycogen, only a small diminution was observed ultrastructurally. Glycogen accumulation, on the other hand, correlates well with increases determined biochemically. alpha-glycogen builds up among lipid droplets, while beta-glycogen concentrates in the cytoplasm of somatic muscle cells paralleling myofibril development. Dense granules, restricted to the lipid body region, are considered as the possible subcellular site for the enzymatic conversion.
在发育中的猪蛔虫幼虫中，甘油三酯的利用与糖原再合成相关。通过每隔2天用淀粉葡萄糖苷酶水解来测定糖原含量，在胚胎发育期间糖原含量从第4天的15%（每毫克干重）急剧下降到第12天的2%。此后，糖原在第20天前增加了3倍，标志着再合成期。甘油三酯脂滴主要在幼虫后半部分被证实，并标记了相互转化的部位。尽管它们是糖原的前体，但在超微结构上仅观察到少量减少。另一方面，糖原积累与生化测定的增加密切相关。α-糖原在脂滴之间积累，而β-糖原集中在体肌细胞的细胞质中，与肌原纤维发育平行。局限于脂质体区域的致密颗粒被认为是酶促转化的可能亚细胞部位。
