Studies on the transmission of simian malaria. VI. Mosquito infection and sporozoite transmission of Plasmodium fragile.

Anopheles b. balabacensis mosquitoes were infected with Plasmodium fragile when fed upon splenectomized Macaca mulatta monkeys. Highest level mosquito infections were obtained when feedings were made from 2 to 4 days prior to the peak in the parasitemia. Transmission to M. mulatta monkeys was obtained via mosquito bite on 2 occasions and via intrahepatic and intravenous inoculation of dissected infected salivary glands on 9 occasions. The prepatent periods ranged from 12 to 17 days with a median of 15 days.