Arfman R C, Loegering D J, Smith J J
Proc Soc Exp Biol Med. 1975 Sep;149(4):1029-31. doi: 10.3181/00379727-149-38950.
In a study of 4-hr hemorrhagic hypotension in dogs, the plasma levels of the lysosomal enzymes, cathepsin (CATH) and acid phosphatase (AP) showed early and progressive increases in activity. The plasma levels of the intestinal fraction of alkaline phosphatase (IAkP) and aspartate aminotransferase (AAT) were increased after 2 hr of hypotension and the liver specific enzyme, ornithine carbamyltransferase (OCT), and creatine phosphokinase (CPK), after 3 hr. All of the enzymes showed large increases after 4 hr of hypotension. The plasma levels of CATH showed the earliest and largest relative increase indicating that with the shock model used, this enzyme was the most sensitive indicator of shock severity. The increase in plasma enzyme levels was probably the result of tissue damage in the splanchnic region but the elevation of plasma CPK, a muscle specific enzyme, indicates some muscle cell damage as well. While the increase in the plasma enzyme activity is probably due, in large part, to cellular damage, it is likely that a decreased enzyme removal rate--resulting from a hypofunctional RES--also contributes to the elevated plasma enzyme levels during hemorrhagic hypotension.
在一项对犬进行4小时失血性低血压的研究中，溶酶体酶组织蛋白酶（CATH）和酸性磷酸酶（AP）的血浆水平显示出活性早期且逐渐升高。碱性磷酸酶肠部分（IAkP）和天冬氨酸氨基转移酶（AAT）的血浆水平在低血压2小时后升高，肝脏特异性酶鸟氨酸氨甲酰基转移酶（OCT）和肌酸磷酸激酶（CPK）在3小时后升高。所有酶在低血压4小时后均大幅升高。CATH的血浆水平显示出最早且最大的相对升高，表明在所使用的休克模型中，该酶是休克严重程度最敏感的指标。血浆酶水平的升高可能是内脏区域组织损伤的结果，但血浆CPK（一种肌肉特异性酶）的升高也表明存在一些肌肉细胞损伤。虽然血浆酶活性的增加很大程度上可能是由于细胞损伤，但在出血性低血压期间，由于网状内皮系统功能减退导致的酶清除率降低也可能导致血浆酶水平升高。
