Harris J W, Bensusan H B
Proc Soc Exp Biol Med. 1975 Sep;149(4):826-9. doi: 10.3181/00379727-149-38907.
1). During the sol to gel transformation of deoxygenated sickle hemoglobin, a time-dependent process preceding gel formation (lag phase) was demonstrated that was inversely proportional to a function of the hemoglobin concentration and that occurred without alteration in temperature, pH, or oxygen tension. 2). As determined by the Schachman modification of the capillary viscometer, preparations of oxyhemoglobin S and A and deoxyhemoglobin A were indistinguishable when compared over a wide range of concentrations. Up to the concentration at which gelling occurred, deoxyhemoglobin S exhibited the same viscosity behavior. The viscosity of deoxygenated hemoglobin S within the lower gelling concentration range was normal during the lag phase and became abnormally high only at the time of gelation.
1). 在脱氧镰状血红蛋白从溶胶到凝胶的转变过程中，证明了在凝胶形成之前存在一个时间依赖性过程（延迟期），该过程与血红蛋白浓度的函数成反比，且在温度、pH值或氧张力无变化的情况下发生。2). 通过毛细管粘度计的沙克曼改良法测定，在很宽的浓度范围内比较时，氧合血红蛋白S和A以及脱氧血红蛋白A的制剂没有区别。直到发生胶凝的浓度，脱氧血红蛋白S表现出相同的粘度行为。在较低胶凝浓度范围内，脱氧血红蛋白S在延迟期的粘度正常，仅在胶凝时才变得异常高。
