Kokorev V S, Podoplekin V D, Pereverzev V D, Fedotova T T
Acta Virol. 1976 Oct;20(5):353-60.
The buoyant densities of Western equine encephalomyelitis virus (an Alphavirus) and tick-borne encephalitis (TBE) virus (a Flavivirus) antigens prepared by different methods were studied. Sucrose density centrifugation revealed a heterogeneity in the density of the virions. The sedimentation pattern and height of peaks of the haemagglutinating activities and infectivity, other conditions being equal, depended both on the virus species and properties of its strains and on the mode of preparation and treatment of the virus-containing material. Different haemagglutinating antigen fractions differed in their capacity to interact with specific antibody. It was suggested that the kinetics of a serological reaction and its result depend on the functional activity of the antigen preparation and, in particular, on the proportion in the reaction mixture of virus particles with a dissimilar antigenic structure.
研究了通过不同方法制备的西部马脑炎病毒（一种甲病毒）和蜱传脑炎（TBE）病毒（一种黄病毒）抗原的浮力密度。蔗糖密度离心显示病毒粒子的密度存在异质性。在其他条件相同的情况下，血凝活性和感染力的沉降模式及峰高既取决于病毒种类及其毒株特性，也取决于含病毒材料的制备和处理方式。不同的血凝抗原组分与特异性抗体相互作用的能力不同。有人提出，血清学反应的动力学及其结果取决于抗原制剂的功能活性，特别是取决于反应混合物中具有不同抗原结构的病毒颗粒的比例。
