Cooper J L, Birchler J A
Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA.
Genesis. 2001 Oct;31(2):64-71. doi: 10.1002/gene.10006.
The reduction in vigor or viability caused by aneuploidy may be the result of trans-acting dosage effects that reduce gene expression. To investigate the molecular and developmental parameters of aneuploid syndromes, the expression of sucrose synthase1 (sus1) and shrunken1 (sh1) was studied in 2-week-old plants. Expression of sus1 and sh1 was first investigated in euploids, where it was found that both transcripts varied in a diurnal fashion. Chromosome arm number can be varied in a series from one to three doses in maize. In the 14 aneuploid dosage series examined, most caused changes in sus1 and sh1 RNA levels that were both gene and tissue specific. Results were compared to previous data from embryo and endosperm tissue. More dosage effects were detected and the magnitude of RNA level modulation was greater in 2-week-old plant tissue. These findings suggest that the molecular consequences of aneuploidy might become more severe as development progresses.
非整倍性导致的活力或生存力下降可能是由于反式作用剂量效应降低了基因表达所致。为了研究非整倍体综合征的分子和发育参数，在两周大的植株中研究了蔗糖合酶1（sus1）和皱缩1（sh1）的表达。首先在整倍体中研究了sus1和sh1的表达，发现这两种转录本均呈现昼夜变化。在玉米中，染色体臂数可以从一个剂量到三个剂量呈系列变化。在所检测的14个非整倍体剂量系列中，大多数导致sus1和sh1 RNA水平发生变化，这些变化具有基因和组织特异性。将结果与先前来自胚胎和胚乳组织的数据进行了比较。在两周大的植物组织中检测到更多的剂量效应，并且RNA水平调节的幅度更大。这些发现表明，随着发育的进行，非整倍性的分子后果可能会变得更加严重。
