Pazo J H, Belforte J E, Barceló A C
Laboratorio de Neurofisiología, Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, 1121, Buenos Aires, Argentina.
Brain Res Brain Res Protoc. 2001 Oct;8(2):132-6. doi: 10.1016/s1385-299x(01)00096-4.
The processing of nociceptive information in the central nervous system has been analysed in most studies by activation of peripheral nerves. However, the limitation of this method is the simultaneous activation of noxious and inocuous fibers. Nevertheless, the stimulation of the tooth pulp is believed to activate mainly nociceptive fibers which could be the method of choice. On the other hand, the response to nociceptive activation of the dental pulp is easily quantified by the amplitude of the jaw opening reflex, a nocifensive flexion withdrawal reflex. In this protocol we describe a technique to manufacture and implant electrodes in lower incisors of the rat and a method to prepare and insert stainless steel twisted bipolar electrodes to record the electromyographic activity of both digastric muscles, in response to nociceptive dental pulp stimulation. This approach was applied in the study of the analgesic effects of the rat's striatal stimulation.
在大多数研究中，中枢神经系统中伤害性信息的处理是通过激活外周神经来分析的。然而，这种方法的局限性在于同时激活了伤害性纤维和无害纤维。尽管如此，牙髓刺激被认为主要激活伤害性纤维，这可能是一种首选方法。另一方面，通过下颌张开反射（一种防御性屈曲退缩反射）的幅度，可以很容易地量化牙髓对伤害性激活的反应。在本实验方案中，我们描述了一种在大鼠下切牙制造并植入电极的技术，以及一种制备并插入不锈钢双股扭绞电极以记录双侧二腹肌肌电活动的方法，用于响应伤害性牙髓刺激。这种方法被应用于大鼠纹状体刺激镇痛作用的研究。
