春季角结膜炎中趋化因子受体的表达
Expression of chemokine receptors in vernal keratoconjunctivitis.
作者信息
Abu El-Asrar A M, Struyf S, Al-Mosallam A A, Missotten L, Van Damme J, Geboes K
机构信息
Department of Ophthalmology, College of Medicine, King Saud University, Riyadh 11411, Saudi Arabia.
出版信息
Br J Ophthalmol. 2001 Nov;85(11):1357-61. doi: 10.1136/bjo.85.11.1357.
BACKGROUND/AIMS: Chemokines are small peptides which are potent activators and chemoattractants for leucocyte subpopulations. Their action is mediated by a family of seven transmembrane spanning G-protein coupled receptors. The aims of this study were to examine the expression of the chemokine receptors CCR1, CCR3, CCR5, CXCR3, and CXCR4 in the conjunctiva of patients with vernal keratoconjunctivitis (VKC) and to investigate the phenotype of inflammatory cells expressing these chemokine receptors.
METHODS
Conjunctival biopsy specimens from 16 patients with active VKC, and eight control subjects were studied by immunohistochemical techniques using a panel of monoclonal antibodies directed against human CCR1, CCR3, CCR5, CXCR3, and CXCR4. The phenotype of inflammatory cells expressing chemokine receptors was examined by double immunohistochemistry.
RESULTS
In the normal conjunctiva, few inflammatory cells expressed CXCR3 in five of eight specimens. There was no immunoreactivity for CCR1, CCR3, CCR5, and CXCR4. In VKC specimens, membranous immunoreactivity for CXCR3 was noted on inflammatory cells in all specimens. Compared with control specimens, VKC specimens showed significantly more inflammatory cells expressing CXCR3 (54.3 (SD 34.3) v 3.3 (5.0); p<0.001). Few CCR1+, CCR3+, CCR5+, and CXCR4+ inflammatory cells were observed in only three of 16 specimens. Double immunohistochemistry revealed that all CXCR3 positive inflammatory cells were CD3 positive T lymphocytes and that 61.7% (3.7%) of the infiltrating T lymphocytes were reactive for CXCR3.
CONCLUSIONS
CXCR3 is the predominant chemokine receptor and is expressed abundantly on T lymphocytes in the conjunctiva of patients with active VKC. These data suggest a potential role for CXCR3 receptors in the regulation of lymphocyte recruitment within conjunctiva of VKC patients. New therapeutic strategies that block CXCR3 may inhibit T lymphocyte recruitment and suppress adverse inflammatory reactions.
背景/目的：趋化因子是一类小分子肽，是白细胞亚群的强效激活剂和趋化剂。它们的作用由一个七次跨膜的G蛋白偶联受体家族介导。本研究的目的是检测春季角结膜炎（VKC）患者结膜中趋化因子受体CCR1、CCR3、CCR5、CXCR3和CXCR4的表达，并研究表达这些趋化因子受体的炎症细胞的表型。
方法
采用一组针对人CCR1、CCR3、CCR5、CXCR3和CXCR4的单克隆抗体，通过免疫组织化学技术研究16例活动性VKC患者和8例对照者的结膜活检标本。通过双重免疫组织化学检测表达趋化因子受体的炎症细胞的表型。
结果
在正常结膜中，8个标本中有5个标本中很少有炎症细胞表达CXCR3。CCR1、CCR3、CCR5和CXCR4无免疫反应性。在VKC标本中，所有标本的炎症细胞上均观察到CXCR3的膜免疫反应性。与对照标本相比，VKC标本中表达CXCR3的炎症细胞明显更多（54.3（标准差34.3）对3.3（5.0）；p<0.001）。在16个标本中只有3个标本中观察到很少的CCR1+、CCR3+、CCR5+和CXCR4+炎症细胞。双重免疫组织化学显示，所有CXCR3阳性炎症细胞均为CD3阳性T淋巴细胞，浸润的T淋巴细胞中有61.7%（3.7%）对CXCR3有反应。
结论
CXCR3是主要的趋化因子受体，在活动性VKC患者结膜的T淋巴细胞上大量表达。这些数据表明CXCR3受体在调节VKC患者结膜内淋巴细胞募集方面可能发挥作用。阻断CXCR3的新治疗策略可能会抑制T淋巴细胞募集并抑制不良炎症反应。
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