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大肠杆菌素A免疫蛋白与大肠杆菌内膜中大肠杆菌素A通道结构域的疏水螺旋发夹相互作用。

Colicin A immunity protein interacts with the hydrophobic helical hairpin of the colicin A channel domain in the Escherichia coli inner membrane.

作者信息

Nardi A, Corda Y, Baty D, Duché D

机构信息

Laboratoire d'Ingénierie des Systèmes Macromoléculaires, Institut de Biologie Structurale et Microbiologie, CNRS, Marseille, France.

出版信息

J Bacteriol. 2001 Nov;183(22):6721-5. doi: 10.1128/JB.183.22.6721-6725.2001.

DOI:10.1128/JB.183.22.6721-6725.2001
PMID:11673448
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC95509/
Abstract

The colicin A pore-forming domain (pfColA) was fused to a bacterial signal peptide (sp-pfColA). This was inserted into the Escherichia coli inner membrane in functional form and could be coimmunoprecipitated with epitope-tagged immunity protein (EpCai). We constructed a series of fusion proteins in which various numbers of sp-pfColA alpha-helices were fused to alkaline phosphatase (AP). We showed that a fusion protein made up of the hydrophobic alpha-helices 8 and 9 of sp-pfColA fused to AP was specifically coimmunoprecipitated with EpCai produced in the same cells. This is the first biochemical evidence that Cai recognizes and interacts with the colicin A hydrophobic helical hairpin.

摘要

将大肠杆菌素A成孔结构域（pfColA）与细菌信号肽（sp-pfColA）融合。它以功能形式插入大肠杆菌内膜，并且可以与表位标签免疫蛋白（EpCai）进行共免疫沉淀。我们构建了一系列融合蛋白，其中将不同数量的sp-pfColAα螺旋与碱性磷酸酶（AP）融合。我们发现，由与AP融合的sp-pfColA的疏水α螺旋8和9组成的融合蛋白与同一细胞中产生的EpCai发生特异性共免疫沉淀。这是关于Cai识别并与大肠杆菌素A疏水螺旋发夹相互作用的首个生化证据。

相似文献

1
Colicin A immunity protein interacts with the hydrophobic helical hairpin of the colicin A channel domain in the Escherichia coli inner membrane.大肠杆菌素A免疫蛋白与大肠杆菌内膜中大肠杆菌素A通道结构域的疏水螺旋发夹相互作用。
J Bacteriol. 2001 Nov;183(22):6721-5. doi: 10.1128/JB.183.22.6721-6725.2001.
2
Integration of the colicin A pore-forming domain into the cytoplasmic membrane of Escherichia coli.将大肠杆菌素A成孔结构域整合到大肠杆菌的细胞质膜中。
J Mol Biol. 1999 Feb 5;285(5):1965-75. doi: 10.1006/jmbi.1998.2423.
3
The C-terminal half of the colicin A pore-forming domain is active in vivo and in vitro.大肠杆菌素A成孔结构域的C端一半在体内和体外均具有活性。
J Mol Biol. 2001 Apr 13;307(5):1293-303. doi: 10.1006/jmbi.2001.4524.
4
The channel domain of colicin A is inhibited by its immunity protein through direct interaction in the Escherichia coli inner membrane.大肠杆菌内膜中，大肠杆菌素A的通道结构域通过与免疫蛋白直接相互作用而受到抑制。
EMBO J. 1996 May 15;15(10):2356-64.
5
The pore-forming domain of colicin A fused to a signal peptide: a tool for studying pore-formation and inhibition.与信号肽融合的大肠菌素A的成孔结构域：一种研究孔形成和抑制的工具。
Biochimie. 2002 May-Jun;84(5-6):455-64. doi: 10.1016/s0300-9084(02)01424-4.
6
The colicin A pore-forming domain fused to mitochondrial intermembrane space sorting signals can be functionally inserted into the Escherichia coli plasma membrane by a mechanism that bypasses the Tol proteins.
Mol Microbiol. 1994 Sep;13(6):1121-31. doi: 10.1111/j.1365-2958.1994.tb00503.x.
7
An alpha-helical hydrophobic hairpin as a specific determinant in protein-protein interaction occurring in Escherichia coli colicin A and B immunity systems.一种α-螺旋疏水发夹结构作为大肠杆菌A和B型大肠杆菌素免疫系统中蛋白质-蛋白质相互作用的特异性决定因素。
J Bacteriol. 1992 Oct;174(20):6432-7. doi: 10.1128/jb.174.20.6432-6437.1992.
8
Crystal structure of colicin Ia.大肠杆菌素Ia的晶体结构。
Nature. 1997 Jan 30;385(6615):461-4. doi: 10.1038/385461a0.
9
A mechanism for toxin insertion into membranes is suggested by the crystal structure of the channel-forming domain of colicin E1.大肠杆菌素E1通道形成结构域的晶体结构提示了毒素插入细胞膜的一种机制。
Structure. 1997 Mar 15;5(3):443-58. doi: 10.1016/s0969-2126(97)00200-1.
10
Uptake across the cell envelope and insertion into the inner membrane of ion channel-forming colicins in E coli.在大肠杆菌中，离子通道形成型大肠杆菌素穿过细胞包膜并插入内膜的过程。
Biochimie. 1990 Feb-Mar;72(2-3):123-30. doi: 10.1016/0300-9084(90)90137-6.

引用本文的文献

1
Channel domain of colicin A modifies the dimeric organization of its immunity protein.类毒素 A 的通道结构域改变了其免疫蛋白的二聚体结构。
J Biol Chem. 2010 Dec 3;285(49):38053-61. doi: 10.1074/jbc.M110.144071. Epub 2010 Oct 4.
2
Colicin biology.大肠杆菌素生物学
Microbiol Mol Biol Rev. 2007 Mar;71(1):158-229. doi: 10.1128/MMBR.00036-06.
3
Integration of a transposon Tn1-encoded inhibitor-resistant beta-lactamase gene, bla(TEM-67) from Proteus mirabilis, into the Escherichia coli chromosome.将来自奇异变形杆菌的转座子Tn1编码的抑制剂抗性β-内酰胺酶基因bla(TEM-67)整合到大肠杆菌染色体中。
Antimicrob Agents Chemother. 2003 Jan;47(1):19-26. doi: 10.1128/AAC.47.1.19-26.2003.

本文引用的文献

1
Identification of specific residues in colicin E1 involved in immunity protein recognition.鉴定大肠杆菌素E1中参与免疫蛋白识别的特定残基。
J Bacteriol. 2001 Mar;183(6):2132-6. doi: 10.1128/JB.183.6.2132-2136.2001.
2
Integration of the colicin A pore-forming domain into the cytoplasmic membrane of Escherichia coli.将大肠杆菌素A成孔结构域整合到大肠杆菌的细胞质膜中。
J Mol Biol. 1999 Feb 5;285(5):1965-75. doi: 10.1006/jmbi.1998.2423.
3
The tip of the hydrophobic hairpin of colicin U is dispensable for colicin U activity but is important for interaction with the immunity protein.大肠杆菌素U疏水发夹的末端对于大肠杆菌素U的活性并非必需，但对于与免疫蛋白的相互作用很重要。
J Bacteriol. 1998 Aug;180(16):4111-5. doi: 10.1128/JB.180.16.4111-4115.1998.
4
A mechanism for toxin insertion into membranes is suggested by the crystal structure of the channel-forming domain of colicin E1.大肠杆菌素E1通道形成结构域的晶体结构提示了毒素插入细胞膜的一种机制。
Structure. 1997 Mar 15;5(3):443-58. doi: 10.1016/s0969-2126(97)00200-1.
5
Major transmembrane movement associated with colicin Ia channel gating.与大肠菌素Ia通道门控相关的主要跨膜运动。
J Gen Physiol. 1996 Mar;107(3):313-28. doi: 10.1085/jgp.107.3.313.
6
The channel domain of colicin A is inhibited by its immunity protein through direct interaction in the Escherichia coli inner membrane.大肠杆菌内膜中，大肠杆菌素A的通道结构域通过与免疫蛋白直接相互作用而受到抑制。
EMBO J. 1996 May 15;15(10):2356-64.
7
The colicin A pore-forming domain fused to mitochondrial intermembrane space sorting signals can be functionally inserted into the Escherichia coli plasma membrane by a mechanism that bypasses the Tol proteins.
Mol Microbiol. 1994 Sep;13(6):1121-31. doi: 10.1111/j.1365-2958.1994.tb00503.x.
8
Intramembrane helix-helix interactions as the basis of inhibition of the colicin E1 ion channel by its immunity protein.膜内螺旋-螺旋相互作用作为大肠杆菌素E1免疫蛋白抑制其离子通道的基础。
J Biol Chem. 1993 May 15;268(14):10176-84.
9
Evidence that the immunity protein inactivates colicin 5 immediately prior to the formation of the transmembrane channel.有证据表明，免疫蛋白在跨膜通道形成之前立即使大肠杆菌素5失活。
J Bacteriol. 1995 Dec;177(23):6966-72. doi: 10.1128/jb.177.23.6966-6972.1995.
10
Identification of a translocated protein segment in a voltage-dependent channel.电压依赖性通道中易位蛋白片段的鉴定
Nature. 1994 Sep 8;371(6493):158-61. doi: 10.1038/371158a0.