Hübner A, Yang X, Nolen D M, Popova T G, Cabello F C, Norgard M V
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12724-9. doi: 10.1073/pnas.231442498.
RpoS and RpoN are two alternative sigma factors typically associated with general stress responses in bacteria. To date, there has been no experimental evidence that RpoS and RpoN can directly control the expression of one another. Herein, using a combined strategy of gene disruption and genetic complementation targeting rpoN and rpoS in Borrelia burgdorferi strain 297, we describe a regulatory network for B. burgdorferi. In this network, RpoN controls the expression of RpoS, which, in turn, governs the expression of two important membrane lipoproteins, outer surface protein C and decorin-binding protein A, and likely other proteins of B. burgdorferi. Our findings provide a foundation for elucidating further key regulatory networks that potentially impact many aspects of B. burgdorferi's parasitic strategy, host range, and virulence expression.
RpoS和RpoN是两种替代的σ因子，通常与细菌的一般应激反应相关。迄今为止，尚无实验证据表明RpoS和RpoN可以直接控制彼此的表达。在此，我们采用基因破坏和基因互补的联合策略，靶向伯氏疏螺旋体菌株297中的rpoN和rpoS，描述了伯氏疏螺旋体的调控网络。在这个网络中，RpoN控制RpoS的表达，而RpoS反过来又调控两种重要的膜脂蛋白——外表面蛋白C和饰胶蛋白结合蛋白A以及伯氏疏螺旋体的其他可能蛋白的表达。我们的研究结果为阐明可能影响伯氏疏螺旋体寄生策略、宿主范围和毒力表达等许多方面的进一步关键调控网络奠定了基础。
