液相色谱/电喷雾离子阱质谱法定量测定血浆中醋酸甲羟孕酮

Quantitative determination of medroxyprogesterone acetate in plasma by liquid chromatography/electrospray ion trap mass spectrometry.

作者信息

Kim S M, Kim D H

机构信息

Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, PO Box 131, Chungryang, Seoul, Korea.

出版信息

Rapid Commun Mass Spectrom. 2001;15(21):2041-5. doi: 10.1002/rcm.478.

DOI:10.1002/rcm.478

PMID:11675672

Abstract

A sensitive and rapid liquid chromatography/electrospray ion trap mass spectrometry (LC/MS/MS) method has been developed for the quantitative determination of medroxyprogesterone acetate (MPA) in human plasma. Plasma samples (1.0 mL) were simply extracted with pentane and the extracts were analyzed by HPLC with the detection of the analyte in the selective reaction monitoring (SRM) mode. The determination of MPA was accurate and reproducible, with a limit of quantitation of 0.05 ng/mL in plasma. The standard calibration curve for MPA was linear (r = 0.998) over the concentration range 0.05-6.0 ng/mL in human plasma. Analysis precision over the concentration range of MPA was lower than 18.8% (relative standard deviation, RSD) and accuracy was between 96.2 and 108.7%.

摘要

已开发出一种灵敏且快速的液相色谱/电喷雾离子阱质谱法（LC/MS/MS），用于定量测定人血浆中的醋酸甲羟孕酮（MPA）。血浆样品（1.0 mL）用戊烷简单萃取，萃取物通过高效液相色谱法进行分析，在选择性反应监测（SRM）模式下检测分析物。MPA的测定准确且可重复，血浆中的定量限为0.05 ng/mL。MPA的标准校准曲线在人血浆0.05 - 6.0 ng/mL浓度范围内呈线性（r = 0.998）。MPA浓度范围内的分析精密度低于18.8%（相对标准偏差，RSD），准确度在96.2%至108.7%之间。