从食用蘑菇虎奶菇新鲜菌核中分离出一种新型核糖体失活蛋白——虎奶菇蛋白。

Isolation of pleuturegin, a novel ribosome-inactivating protein from fresh sclerotia of the edible mushroom Pleurotus tuber-regium.

作者信息

Wang H X, Ng T B

机构信息

Department of Microbiology, College of Biological Science, China Agricultural University, Beijing, China.

出版信息

Biochem Biophys Res Commun. 2001 Nov 2;288(3):718-21. doi: 10.1006/bbrc.2001.5816.

DOI:10.1006/bbrc.2001.5816

PMID:11676502

Abstract

A ribosome-inactivating protein (RIP) designated pleuturegin, which inhibited translation in a cell-free rabbit reticulocyte lysate system with an IC50 of 0.5 nM, was purified from fresh sclerotia of the edible mushroom Pleurotus tuber-regium. Pleuturegin was distinguished from most plant and previously reported mushroom RIPs in that it was adsorbed on DEAE-cellulose and unadsorbed on SP-Sepharose, although all of them were adsorbed on Affi-gel blue gel. Pleuturegin demonstrated an N-terminal sequence that was different from those of RIPs from Flammulina velutipes (flammulin and velutin), Hypsizygus marmoreus (hypsin), and Lyophyllum shimeji (lyophyllin), the only mushroom RIPs with known N-terminal sequences. The molecular mass of pleuturegin was 38 kDa, similar to that of flammulin (40 kDa) but considerably larger than those of velutin (13.8 kDa), hypsin (20 kDa), and lyophyllin (20 kDa). Pleuturegin was devoid of ribonuclease activity.

摘要

一种名为侧耳菌素的核糖体失活蛋白（RIP），在无细胞兔网织红细胞裂解液系统中抑制翻译，其半数抑制浓度（IC50）为0.5 nM，该蛋白是从食用菌虎奶菇的新鲜菌核中纯化得到的。侧耳菌素与大多数植物及先前报道的蘑菇RIP不同，尽管它们都能吸附在Affi - 凝胶蓝胶上，但侧耳菌素能吸附在DEAE - 纤维素上，而不吸附在SP - 琼脂糖上。侧耳菌素的N端序列与金针菇（flammulin和velutin）、真姬菇（hypsin）和茶树菇（lyophyllin）的RIP不同，这几种是仅有的已知N端序列的蘑菇RIP。侧耳菌素的分子量为38 kDa，与flammulin（40 kDa）相似，但比velutin（13.8 kDa）、hypsin（20 kDa）和lyophyllin（20 kDa）大得多。侧耳菌素没有核糖核酸酶活性。