Möller P, Taugner R
Pflugers Arch. 1975;354(3):241-8. doi: 10.1007/BF00584647.
The intrarenal distribution of radioiodinated human serum albumin (125RISA) after intravenous injection was studied in Syrian hamsters by scintillation counting and frozen section autoradiography. After 15, 30 and 60 min the virtual plasma albumin space in the renal cortex of the hamster represented 6.49, 7.13, and 8.06 percent respectively of the kidney tissue volume. From the cortex to the renal papilla the albumin space increased to about 30 percent of the tissue volume. In comparison to this the albumin space in the renal cortex of the rat was about 20% and in the renal papilla about 33% (11). Frozen section autoradiography indicated that the distribution of radioalbumin in the renal cortex of the Syrian hamster is limited mainly to the kidney vessels, being especially noticeable in the glomerular capillaries. Toward the papilla increasingly greater (mainly extratubular) activity could be observed not only intravascularly but also interstitially. In the cortex of the rat kidney, on the other hand, radioactive albumin was accumulated (probably by filtration and reabsorption) predominantly in the proximal tubular epithelium. Wtithin 30 min thekidneys of the rat excreted mor than 10 times as much 125I than the hamster kidneys. These results (substantially less cortical accumulation and urinary excretion of radioalbumin in the Syrian hamster) indicate that, in contrast to the rat, obviously much less albumin is filtered (and then accumulated by proximal reabsorption) by the Syrian hamster glomeruli. This suggests that the Syrian hamster kidney is more suitable than the rat kidney for determining the interstitial, cortical, albumin space.
通过闪烁计数和冰冻切片放射自显影术，研究了静脉注射放射性碘化人血清白蛋白（¹²⁵RISA）后在叙利亚仓鼠体内的肾内分布情况。注射后15、30和60分钟，仓鼠肾皮质中的虚拟血浆白蛋白空间分别占肾组织体积的6.49%、7.13%和8.06%。从皮质到肾乳头，白蛋白空间增加到组织体积的约30%。相比之下，大鼠肾皮质中的白蛋白空间约为20%，肾乳头中约为33%（11）。冰冻切片放射自显影表明，叙利亚仓鼠肾皮质中放射性白蛋白的分布主要局限于肾血管，在肾小球毛细血管中尤为明显。朝着乳头方向，不仅在血管内而且在间质中都能观察到越来越高的（主要是肾小管外的）活性。另一方面，在大鼠肾皮质中，放射性白蛋白主要在近端肾小管上皮中积累（可能是通过滤过和重吸收）。在30分钟内，大鼠肾脏排泄的¹²⁵I比仓鼠肾脏多10倍以上。这些结果（叙利亚仓鼠中放射性白蛋白的皮质积累和尿排泄明显较少）表明，与大鼠相比，叙利亚仓鼠肾小球滤过的白蛋白明显少得多（然后通过近端重吸收积累）。这表明，叙利亚仓鼠肾比大鼠肾更适合用于确定间质、皮质白蛋白空间。
