Kambhampati D, Nielsen P E, Knoll W
Max-Planck-Institut für Polymerforschung, Postfach 3148, Ackermannweg 10, 55128 Mainz, Germany.
Biosens Bioelectron. 2001 Dec;16(9-12):1109-18. doi: 10.1016/s0956-5663(01)00239-1.
Plasmon surface polaritons, resonantly excited in the Kretschmann format, are used to enhance the fluorescence emission of chromophore-labeled oligonucleotides (15mers) binding to surface-attached (via biotin-streptavidin linkages) complement catcher probes. A detailed analysis of the association and dissociation kinetics as well as the affinity constants is given for a mismatch 1 hybrid, emphasizing, in particular, the experimental conditions that are required to allow for an artifact-free determination of rate constants. A first comparison between DNA- and peptide nucleic acid (PNA-) probes shows similar affinities, however, significant deviations from single-exponential kinetics predicted by a simple Langmuir model for the PNA case are found.
以Kretschmann模式共振激发的表面等离激元极化子，用于增强与表面附着（通过生物素-链霉亲和素连接）的互补捕获探针结合的发色团标记寡核苷酸（15聚体）的荧光发射。对错配1杂交体的缔合和解离动力学以及亲和常数进行了详细分析，特别强调了为无假象地测定速率常数所需的实验条件。DNA探针和肽核酸（PNA）探针的首次比较显示出相似的亲和力，然而，发现PNA的情况与简单朗缪尔模型预测的单指数动力学存在显著偏差。
