Razumova I V
Med Parazitol (Mosk). 2001 Jul-Sep(3):16-22.
A method has been developed for lifetime determination of the physiological age of hungry imagoes on Ixodes and Dermacentor ticks of natural populations. The procedure has been used to study an alive tick in the liquid (water, physiological saline) against the light background. In doing so, some previously described anatomic and age-specific signs of Ixodes are imaged outwards and their overall age-specific signs are employed. The author has proposed the following criteria for determination of the age of ticks: appearance: 1) the body's thickness; 2) the wrinkles and colour of the cuticle, and the combined sign; 3) transcuticular visibility of the viscera. The last sign was earlier found [8, 9], which is basic in the new method. There is a significant generic specificity of its manifestation. Tables have been constructed for determining four main physiological ages (I-IV) of ticks of both genera. The method has been applied to the study of natural populations of I. persulcatus, D. reticulatus and D. marginatus in combination with virological studies. The method developed allows one to proceed to make a mass determination of the physiological age of alive ticks and basically expands the potentialities of its application.
已开发出一种用于确定自然种群中饥饿成年硬蜱（硬蜱属和血蜱属）生理年龄的寿命测定方法。该程序用于在液体（水、生理盐水）中、以浅色背景研究活蜱。在此过程中，硬蜱一些先前描述的解剖学和年龄特异性特征会向外成像，并采用其整体年龄特异性特征。作者提出了以下确定蜱虫年龄的标准：外观：1）身体厚度；2）角质层的皱纹和颜色以及综合特征；3）内脏透过角质层的可见度。最后一个特征是之前发现的[8,9]，它是新方法的基础。其表现存在显著的属特异性。已构建表格来确定这两个属蜱虫的四个主要生理年龄（I-IV）。该方法已结合病毒学研究应用于全沟硬蜱、网纹血蜱和边缘血蜱自然种群的研究。所开发的方法能够对活蜱的生理年龄进行大规模测定，并从根本上扩展了其应用潜力。
