Baumgartner U, Baier P, Mappes H J, Farthmann E H
Department of Surgery, University of Freiburg, Germany.
Dig Dis Sci. 2001 Oct;46(10):2098-103. doi: 10.1023/a:1011986125758.
The aim of this study was to analyze whether velocity of periportal and pericentral translocation is different for bile acids differing in their physicochemical properties. Isolated livers of male Sprague-Dawley rats were perfused antegradely or retrogradely, in single pass arrangement with labeled taurodehydrocholate, cholate, taurocholate, glycocholate, or taurodeoxycholate (inflow rate: 32 nmol/min/g liver). Bile was collected at 2-min intervals; aliquots were counted in a liquid scintillation spectrophotometer to trace uptake rate and biliary excretion profile. Biliary excretion patterns of all bile acids tested were almost identical in periportal cells; in contrast, it differed greatly in pericentral cells. Pericentral cells excreted taurodehydrocholate as fast as periportal cells, while periportal cells eliminated taurodeoxycholate about four times as fast and cholate, taurocholate and cholate about two times as fast as pericentral cells. It is concluded that, in contrast to periportal translocation, the velocity of pericentral translocation depends upon the hydrophilicity of the respective bile acid. Therefore, different or additional translocation pathways for bile acids may be involved in periportal versus pericentral cells.
本研究的目的是分析物理化学性质不同的胆汁酸在门静脉周围和中央周围转运速度是否存在差异。将雄性Sprague-Dawley大鼠的离体肝脏进行顺行或逆行灌注，以单次通过的方式灌注标记的牛磺去氢胆酸盐、胆酸盐、牛磺胆酸盐、甘氨胆酸盐或牛磺脱氧胆酸盐（流入速率：32 nmol/min/g肝脏）。每隔2分钟收集胆汁；取等分试样在液体闪烁分光光度计中计数，以追踪摄取率和胆汁排泄情况。在门静脉周围细胞中，所有测试胆汁酸的胆汁排泄模式几乎相同；相反，在中央周围细胞中差异很大。中央周围细胞排泄牛磺去氢胆酸盐的速度与门静脉周围细胞一样快，而门静脉周围细胞消除牛磺脱氧胆酸盐的速度约为中央周围细胞的四倍，胆酸盐、牛磺胆酸盐和甘氨胆酸盐的消除速度约为中央周围细胞的两倍。得出的结论是，与门静脉周围转运不同，中央周围转运的速度取决于相应胆汁酸的亲水性。因此，门静脉周围细胞与中央周围细胞中可能涉及不同的或额外的胆汁酸转运途径。
