Taniguchi N, Ekuni A, Ko J H, Miyoshi E, Ikeda Y, Ihara Y, Nishikawa A, Honke K, Takahashi M
Department of Biochemistry, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
Proteomics. 2001 Feb;1(2):239-47. doi: 10.1002/1615-9861(200102)1:2<239::AID-PROT239>3.0.CO;2-K.
The transfection of glycoprotein glycosyltransferase genes into cells leads to modification of both the structure and function of the glycoproteins and as a result, changes in glycome patterns. N-glycan branching enzymes hold some promise as a model system for the identification of glycome patterns. Both N-acetylglucosaminyltransferase III and alpha 1-6 fucosyltransferase are typical glycosyltransferases, which are involved in the branching of N-glycans. The resulting enzymatic products, bisecting N-GlcNAc and alpha 1-6 fucose residues, are no longer modified by other glycosyltransferases and it is a relatively simple task to identify their modification by means of lectins. In this review, the glycome patterns of glycosyltransferase gene transfectants and the non-transfectants were compared by two-dimensional gel electrophoresis and lectin staining, and the biological significance of the two genes are described. Analyses of glycome patterns by transfecting glycosyltransferase genes will lead to new fields of study in the area of postgenome research.
将糖蛋白糖基转移酶基因转染到细胞中会导致糖蛋白的结构和功能发生改变，从而使糖组模式发生变化。N-聚糖分支酶有望成为识别糖组模式的模型系统。N-乙酰葡糖胺转移酶III和α1-6岩藻糖基转移酶都是典型的糖基转移酶，它们参与N-聚糖的分支。产生的酶促产物，即平分型N-乙酰葡糖胺和α1-6岩藻糖残基，不再被其他糖基转移酶修饰，通过凝集素鉴定它们的修饰相对简单。在本综述中，通过二维凝胶电泳和凝集素染色比较了糖基转移酶基因转染细胞和未转染细胞的糖组模式，并描述了这两个基因的生物学意义。通过转染糖基转移酶基因分析糖组模式将在后基因组研究领域开辟新的研究方向。
