Twerenbold D, Gerber D, Gritti D, Gonin Y, Netuschill A, Rossel F, Schenker D, Vuilleumier J L
Institut de Physique, Université Neuchâtel, Switzerland.
Proteomics. 2001 Jan;1(1):66-9. doi: 10.1002/1615-9861(200101)1:1<66::AID-PROT66>3.0.CO;2-S.
Experimental results from equimolar PEG and protein standards samples are presented from a MALDI-TOF mass spectrometer equipped with both ionizing detectors and the novel single molecule sensitive cryodetectors. The data are consistent with a model hypothesis suggesting that the observed decrease in signal strength in conventional ionizing detector MALDI-TOF mass spectrometers can be explained by the exponentially decreasing quantum efficiency of ionizing detectors. Cryodetectors, in contrast, have a mass independent detection efficiency of 100% on impact and provide additional information on the molecule state owing to the calorimetric nature of the detection mechanism.
给出了等摩尔聚乙二醇（PEG）和蛋白质标准样品的实验结果，这些结果来自一台配备了电离探测器和新型单分子灵敏低温探测器的基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱仪。数据与一个模型假设相符，该假设表明，传统电离探测器MALDI-TOF质谱仪中观察到的信号强度下降可以用电离探测器量子效率呈指数下降来解释。相比之下，低温探测器在撞击时具有100%的质量独立检测效率，并且由于检测机制的量热性质，能提供有关分子状态的额外信息。
