Zhang S, Wu X Y, Li Y H, Zhang X Y
Department of Aerospace Medicine, The Fourth Military Medical University, Xi' an 710032.
Space Med Med Eng (Beijing). 2001 Aug;14(4):235-9.
To observe the effects of simulated weightlessness on the release of prostaglandin E2 in rat calvarial osteoblasts by flow shear stress (FSS).
Osteoblasts were isolated from neonatal rat calvariae and then were set to two groups. One was cultured in 1 G terrestrial gravitational environment. The other was cultured on a clinostat (simulating weightlessness environment) for 60 h, then the osteoblasts were treated with 0.5 Pa or 1.5 Pa FSS in a flow chamber for 1 h. Then release of PGE2 in the osteoblasts was determined.
0.5 Pa and 1.5 Pa FSS treatments significantly increased the release of PGE2 in osteoblasts cultured in 1 G environment (P < 0.01) , but there was no remarkable difference between the responses of 0.5 Pa FSS treatment group and 1.5 Pa group (P > 0.05). By contrast, in cells cultured in simulated weightlessness environment, no detectable release of PGE2 was found with the treatment of 0.5 Pa FSS (P < 0.01). The release of PGE2 was delayed and the amount of PGE2 Production was remarkably decreased with 1.5 Pa FSS treatment (P < 0.01).
These results demonstrated that the responsiveness of osteoblasts to FSS was down-regulated in simulated weightlessness.
观察模拟失重对大鼠颅骨成骨细胞中前列腺素E2经流动剪切力（FSS）释放的影响。
从新生大鼠颅骨分离成骨细胞，然后分为两组。一组在1G地球重力环境中培养。另一组在回转器上培养（模拟失重环境）60小时，然后将成骨细胞在流动腔中用0.5Pa或1.5Pa的FSS处理1小时。然后测定成骨细胞中PGE2的释放。
0.5Pa和1.5Pa的FSS处理显著增加了在1G环境中培养的成骨细胞中PGE2的释放（P<0.01），但0.5Pa FSS处理组和1.5Pa组的反应之间没有显著差异（P>0.05）。相比之下，在模拟失重环境中培养的细胞中，用0.5Pa FSS处理未检测到PGE2的释放（P<0.01）。用1.5Pa FSS处理时，PGE2的释放延迟且PGE2产生量显著降低（P<0.01）。
这些结果表明，在模拟失重状态下，成骨细胞对FSS的反应性下调。
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