整合了限制性片段长度多态性（RFLP）、简单序列重复（SSR）、随机扩增多态性DNA（RAPD）和形态学标记的桃连锁图谱。

A peach linkage map integrating RFLPs, SSRs, RAPDs, and morphological markers.

作者信息

Dettori M T, Quarta R, Verde I

机构信息

Istituto Sperimentale per la Frutticoltura, Rome, Italy.

出版信息

Genome. 2001 Oct;44(5):783-90.

PMID:11681601

Abstract

A linkage map was obtained using a BC1 progeny (Prunus persica x (P. persica x P ferganensis)). The map is composed of 109 loci (74 RFLPs, 17 SSRs, 16 RAPDs, and two morphological traits) distributed in 10 linkage groups. Loci, segregating in five different ratios, were integrated in the map with JoinMap 2.0 software. The map covers 521 cM of the peach genome. The average distance between adjacent loci is 4.8 cM. Two monogenic traits, flesh adhesion (F/f) and leaf glands (E/e), were placed on the map. Thirty-two loci in common with a saturated linkage map of Prunus allowed a comparative analysis to be made between the two maps. Homologies were found among the respective linkage groups. No relevant differences were observed in the linear order of the common loci.

摘要

利用一个BC1后代（桃×（桃×费尔干纳桃））构建了一个连锁图谱。该图谱由分布在10个连锁群中的109个位点（74个RFLP、17个SSR、16个RAPD和两个形态性状）组成。以5种不同比例分离的位点通过JoinMap 2.0软件整合到图谱中。该图谱覆盖了桃基因组的521厘摩。相邻位点之间的平均距离为4.8厘摩。两个单基因性状，果肉粘连（F/f）和叶腺体（E/e），被定位到图谱上。与李属饱和连锁图谱共有32个位点，从而可以对这两个图谱进行比较分析。在各自的连锁群之间发现了同源性。在共有位点的线性顺序上未观察到相关差异。