Diaz-Torres M L, Russell R R
Oral Biology, Dental School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4BW, UK.
FEMS Microbiol Lett. 2001 Oct 16;204(1):23-8. doi: 10.1111/j.1574-6968.2001.tb10856.x.
A homologue of the HtrA family of stress-response proteases was detected by analysis of the Streptococcus mutans genome sequence. Disabling of the S. mutans htrA gene by insertional inactivation resulted in bacterial clumping in liquid medium, altered colony morphology and a reduced ability to withstand high temperature, extremes of pH or oxidative stress. Seven different extracellular or wall-associated proteins that are known to be subject to post-translational proteolysis were examined in cultures of wild-type S. mutans and an htrA mutant. Inactivation of the htrA protease had no effect on degradation of the proteins.
通过对变形链球菌基因组序列分析，检测到应激反应蛋白酶HtrA家族的一个同源物。通过插入失活使变形链球菌htrA基因失活，导致细菌在液体培养基中聚集、菌落形态改变以及耐受高温、极端pH值或氧化应激的能力降低。在野生型变形链球菌和htrA突变体培养物中检测了七种已知会进行翻译后蛋白水解的不同细胞外或壁相关蛋白。htrA蛋白酶失活对这些蛋白质的降解没有影响。
