Ausmees N, Mayer R, Weinhouse H, Volman G, Amikam D, Benziman M, Lindberg M
Department of Microbiology, Swedish University of Agricultural Sciences, SLU, Box 7025, S-75007 Uppsala, Sweden.
FEMS Microbiol Lett. 2001 Oct 16;204(1):163-7. doi: 10.1111/j.1574-6968.2001.tb10880.x.
A conserved domain, called GGDEF (referring to a conserved central sequence pattern), is detected in many procaryotic proteins, often in various combinations with putative sensory-regulatory components. Most sequenced bacterial genomes contain several different GGDEF proteins. The function of this domain has so far not been experimentally shown. Through genetic complementation using genes from three different bacteria encoding proteins with GGDEF domains as the only element in common, we present genetic data indicating (a) that the GGDEF domain is responsible for the diguanylate cyclase activity of these proteins, and (b) that the activity of cellulose synthase in Rhizobium leguminosarum bv. trifolii and Agrobacterium tumefaciens is regulated by cyclic di-GMP as in Acetobacter xylinum.
在许多原核生物蛋白质中检测到一个保守结构域，称为GGDEF（指保守的中央序列模式），它常与假定的传感调节成分以各种组合形式存在。大多数已测序的细菌基因组含有几种不同的GGDEF蛋白。到目前为止，该结构域的功能尚未通过实验得到证实。通过使用来自三种不同细菌的基因进行遗传互补，这些基因编码的蛋白仅以GGDEF结构域作为共同元素，我们提供的遗传数据表明：（a）GGDEF结构域负责这些蛋白的二鸟苷酸环化酶活性；（b）豌豆根瘤菌三叶草生物型和根癌土壤杆菌中纤维素合酶的活性如同木醋杆菌一样受环二鸟苷酸调控。
