Okatani A T, Uto T, Taniguchi T, Horisaka T, Horikita T, Kaneko K, Hayashidani H
Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
J Clin Microbiol. 2001 Nov;39(11):4032-6. doi: 10.1128/JCM.39.11.4032-4036.2001.
We report here the first analysis of Erysipelothrix spp. using pulsed-field gel electrophoresis (PFGE). Seventy strains of Erysipelothrix spp. were analyzed. SmaI, AscI, and NotI were tested for the ability to cleave the DNA extracted from those strains, and among them, SmaI was the most reliable enzyme. Sixty-three distinct PFGE patterns were produced, and no DNA degradation was observed, allowing the identification of all of the strains. Based on these results and on those of a previous analysis using randomly amplified polymorphic DNA and ribotyping, PFGE with SmaI might be considered to be more sensitive than those methods and to be the best method for epidemiological studies of strains of this genus.
我们在此报告首次使用脉冲场凝胶电泳(PFGE)对红斑丹毒丝菌属进行的分析。对70株红斑丹毒丝菌属菌株进行了分析。测试了SmaI、AscI和NotI对从这些菌株中提取的DNA的切割能力,其中SmaI是最可靠的酶。产生了63种不同的PFGE图谱,未观察到DNA降解,从而能够鉴定所有菌株。基于这些结果以及先前使用随机扩增多态性DNA和核糖体分型分析的结果,使用SmaI的PFGE可能被认为比那些方法更敏感,并且是该属菌株流行病学研究的最佳方法。