Pañeda C, Villar A V, Alonso A, Goñi F M, Varela F, Brodbeck U, León Y, Varela-Nieto I, Jones D R
Instituto de Investigaciones Biomédicas Alberto Sols (C.S.I.C.), Madrid, Spain.
Mol Med. 2001 Jul;7(7):454-60.
Signal transduction through the hydrolysis of glycosyl-phosphatidylinositol (GPI) leading to the release of the water-soluble inositol phosphoglycan (IPG) molecules has been demonstrated to be important for mediating some of the actions of insulin and insulin-like growth factor-I (IGF-I).
In the present study, GPI from grass pea (Lathyrus sativus) seeds has been purified and partially characterized on the basis of its chromatographic properties and its compositional analysis.
The results indicate that it shows similarities to GPI previously isolated from other sources such as rat liver. IPG was generated from L. sativus seed GPI by hydrolysis with a GPI-specific phospholipase D (GPI-PLD). This IPG inhibited protein kinase A (PKA) in an in vitro assay, caused cell proliferation in explanted cochleovestibular ganglia (CVG), and decreased 8-Br-cAMP-induced phosphoenolpyruvate carboxykinase (PEPCK) mRNA expression in cultured hepatoma cells.
Our data indicate that L. sativus seed IPG possess insulin-mimetic activities. This may explain why L. sativus seeds have been used in some traditional medicines to ameliorate diabetic symptoms.
通过糖基磷脂酰肌醇（GPI）水解导致水溶性肌醇磷酸聚糖（IPG）分子释放的信号转导已被证明对介导胰岛素和胰岛素样生长因子-I（IGF-I）的某些作用很重要。
在本研究中，已从草豌豆（Lathyrus sativus）种子中纯化了GPI，并根据其色谱特性和成分分析对其进行了部分表征。
结果表明，它与先前从其他来源（如大鼠肝脏）分离的GPI具有相似性。通过用GPI特异性磷脂酶D（GPI-PLD）水解从草豌豆种子GPI中产生IPG。这种IPG在体外试验中抑制蛋白激酶A（PKA），在外植的耳蜗前庭神经节（CVG）中引起细胞增殖，并降低培养的肝癌细胞中8-溴-cAMP诱导的磷酸烯醇丙酮酸羧激酶（PEPCK）mRNA表达。
我们的数据表明草豌豆种子IPG具有胰岛素模拟活性。这可能解释了为什么草豌豆种子在一些传统药物中被用于改善糖尿病症状。
