Scholl Y, Höke D, Dräger B
Institut für Pharmazeutische Biologie, Martin-Luther-Universität Halle-Wittenberg, Hoher Weg 8, 06120, Halle/Saale, Germany.
Phytochemistry. 2001 Nov;58(6):883-9. doi: 10.1016/s0031-9422(01)00362-4.
Calystegines were measured in roots and aerial parts of Calystegia sepium. The accumulation appears developmentally regulated. Calystegine accumulation in hairy root cultures follows growth and reaches maximal values of 1,5 mg/g dry mass. 15N-Labelled tropinone was fed to root cultures and the incorporation of label into calystegines and further metabolites of the tropane alkaloid pathway was measured after 2, 4 and 6 days. Pseudotropine was completely labelled after 2 days, and calystegine A(3) was labelled faster than the calystegines of the B-group. 2,7-Dihydroxynortropane also incorporated 15N from tropinone and is suggested to be a by-product of the tropane alkaloid pathway leading to calystegines.
对旋花属植物打碗花的根和地上部分的卡利西平进行了测定。其积累似乎受发育调控。发根培养物中卡利西平的积累随生长而增加，达到干重1.5毫克/克的最大值。将15N标记的托品酮加入到根培养物中，并在2天、4天和6天后测定标记物掺入卡利西平和托烷生物碱途径的其他代谢产物中的情况。2天后假托品完全被标记，卡利西平A(3)的标记速度比B组的卡利西平快。2,7-二羟基去甲托烷也从托品酮中掺入了15N，被认为是通向卡利西平的托烷生物碱途径的副产物。
