非乳头状鼻黏膜、内生性鼻鼻窦乳头状瘤及相关癌中p21（waf1/cip1）的表达模式
Patterns of p21(waf1/cip1) expression in non-papillomatous nasal mucosa, endophytic sinonasal papillomas, and associated carcinomas.
作者信息
Schwerer M J, Sailer A, Kraft K, Baczako K, Maier H
机构信息
Department of Pathology, Military Hospital Ulm, D-89081 Ulm/Donau, Germany.
出版信息
J Clin Pathol. 2001 Nov;54(11):871-6. doi: 10.1136/jcp.54.11.871.
AIMS
To clarify p21(waf1/cip1) expression in sinonasal lesions.
METHODS
Archived surgical specimens from 38 patients were investigated by means of immunohistochemistry. p21(waf1/cip1) staining was evaluated in the different layers of the epithelium. In addition, human papillomavirus (HPV) infection and p53 protein overexpression were assessed and correlated with p21(waf1/cip1) expression.
RESULTS
p21(waf1/cip1) staining was negative in non-papillomatous nasal mucosa. HPV infection and p53 protein overexpression were not seen. Sixteen of 20 inverted papillomas showed p21(waf1/cip1) expression. HPV infection was found in 16 cases and p53 protein overexpression was present in 13 specimens. Expression of p21(waf1/cip1) was restricted to surface cells in five cases, but involved basal/parabasal cells in 11 specimens. Immunoreactivity for p21(waf1/cip1) in basal/parabasal cells colocalised with p53 protein overexpression. Enhanced expression rates for p21(waf1/cip1) were seen in transitional and squamous epithelium compared with columnar epithelium. p21(waf1/cip1) expression involved only surface cells in cylindrical cell papillomas. HPV infection and p53 protein overexpression were detected in all specimens. One of five squamous cell carcinomas showed p21(waf1/cip1) expression. HPV infection was seen in two cases, and all carcinomas showed p53 protein overexpression.
CONCLUSIONS
Expression of p21(waf1/cip1) is associated with terminal differentiation in surface cells in inverted papillomas and cylindrical cell papillomas, but not in non-papillomatous nasal mucosa. Overexpression of p53 protein colocalises with p21(waf1/cip1) expression in basal/parabasal cells in inverted papillomas but not in cylindrical cell papillomas. Expression of p21(waf1/cip1) in squamous cell carcinomas involves a subset of tumours with p53 protein overexpression.
目的
阐明p21（waf1/cip1）在鼻窦病变中的表达情况。
方法
采用免疫组织化学方法对38例患者的存档手术标本进行研究。在不同上皮层评估p21（waf1/cip1）染色情况。此外，评估人乳头瘤病毒（HPV）感染和p53蛋白过表达情况，并将其与p21（waf1/cip1）表达进行关联分析。
结果
在非乳头状鼻黏膜中，p21（waf1/cip1）染色呈阴性。未发现HPV感染和p53蛋白过表达。20例内翻性乳头状瘤中有16例显示p21（waf1/cip1）表达。16例发现HPV感染，13例标本存在p53蛋白过表达。5例p21（waf1/cip1）表达局限于表面细胞，但11例标本累及基底/副基底细胞。基底/副基底细胞中p21（waf1/cip1）的免疫反应性与p53蛋白过表达共定位。与柱状上皮相比，在移行上皮和鳞状上皮中p21（waf1/cip1）表达率升高。在柱状细胞乳头状瘤中，p21（waf1/cip1）表达仅累及表面细胞。所有标本均检测到HPV感染和p53蛋白过表达。5例鳞状细胞癌中有1例显示p21（waf1/cip1）表达。2例发现HPV感染，所有癌均显示p53蛋白过表达。
结论
p21（waf1/cip1）表达与内翻性乳头状瘤和柱状细胞乳头状瘤表面细胞的终末分化相关，但与非乳头状鼻黏膜无关。在倒生性乳头状瘤的基底/副基底细胞中，p53蛋白过表达与p21（waf1/cip1）表达共定位，但在柱状细胞乳头状瘤中不共定位。鳞状细胞癌中p21（waf1/cip1）表达涉及p53蛋白过表达的一部分肿瘤。
Zotero 插件