黑猩猩体内不同表型变异的HIV-1和SIV（cpz）早期病毒载量的差异。

Differences in early virus loads with different phenotypic variants of HIV-1 and SIV(cpz) in chimpanzees.

作者信息

ten Haaft P, Murthy K, Salas M, McClure H, Dubbes R, Koornstra W, Niphuis H, Davis D, van der Groen G, Heeney J

机构信息

Department of Virology, Biomedical Primate Research Centre, Rijswijk, the Netherlands.

出版信息

AIDS. 2001 Nov 9;15(16):2085-92. doi: 10.1097/00002030-200111090-00003.

DOI:10.1097/00002030-200111090-00003

PMID:11684927

Abstract

OBJECTIVE

A comparative study of the replication kinetics of different HIV-1 variants (including SIV(cpz)) was undertaken to determine which viral characteristics were associated with sustained plasma viraemia in chimpanzees.

DESIGN

Plasma samples from chimpanzees infected with six different HIV-1 clade B isolates were compared with plasma samples from SIV(cpz-ant)-infected chimpanzees.

METHODS

A pan-clade quantitative competitive reverse transcriptase-polymerase chain reaction assay was developed based on conserved primer sequences recognizing M, N and O human lentiviruses as well as different SIV(cpz) isolates.

RESULTS

Important differences between early kinetics in the human lentivirus isolates as well as compared with the chimpanzee isolate SIV(cpz-ant) were observed. R5-dependent non-syncytium-inducing (NSI) isolates (5016, Ba-L, SIV(cpz)) were found to have relatively higher viral loads than the syncytium-inducing (SI), X4-dependent primary (SF2), T cell-adapted (IIIB) or X4/R5 (Han2, DH12) SI primary isolates.

CONCLUSION

Infection of chimpanzees with NSI R5-utilizing isolates correlated with persistent viraemia (approximately 10(4) RNA equivalents/ml) in contrast to transient viraemia observed after infection with SI X4-utilizing isolates.

摘要

目的

对不同HIV-1变体（包括SIV（cpz））的复制动力学进行比较研究，以确定哪些病毒特征与黑猩猩体内持续的血浆病毒血症相关。

设计

将感染六种不同HIV-1 B亚型分离株的黑猩猩的血浆样本与感染SIV（cpz-ant）的黑猩猩的血浆样本进行比较。

方法

基于识别M、N和O型人类慢病毒以及不同SIV（cpz）分离株的保守引物序列，开发了一种泛亚型定量竞争性逆转录-聚合酶链反应检测方法。

结果

观察到人类慢病毒分离株以及与黑猩猩分离株SIV（cpz-ant）相比在早期动力学方面存在重要差异。发现依赖R5的非合胞体诱导（NSI）分离株（5016、Ba-L、SIV（cpz））的病毒载量相对高于合胞体诱导（SI）、依赖X4的原代（SF2）、T细胞适应型（IIIB）或X4/R5（Han2、DH12）SI原代分离株。