Vanden Haesevelde M M, Peeters M, Jannes G, Janssens W, van der Groen G, Sharp P M, Saman E
Innogenetics N.V., Industriepark 7, Gent, Belgium.
Virology. 1996 Jul 15;221(2):346-50. doi: 10.1006/viro.1996.0384.
Two strains of simian immunodeficiency viruses (SIV) isolated from chimpanzees (SIVCPZ-GAB and SIVCPZ-GAB2) originating from Gabon have previously been genetically characterized and shown to belong phylogenetically to the same lineage as the human immunodeficiency virus type 1 (HIV-1). We describe the sequence analysis of a third HIV-1-related virus, SIVCPZ-ANT, isolated from a wild captured chimpanzee originating from Zaire. This virus displayed the same genetic organization as HIV-1 and was found to fall on the same lineage as HIV-1 and SIVCPZ-GAB. Protein sequence identity with SIVCPZ-GAB ranged from 72% (Pol) to 48% (Env) for the structural proteins, while a particularly divergent Vpu was found (only 25% identity to SIVCPZ-GAB). The V3 regions of the SIVCPZ isolates were exceptionally conserved in contrast to the high divergence of V3 among HIV-1 isolates. However, SIVCPZ-ANT did not show a greater degree of sequence similarity with SIVCPZ-GAB than with HIV-1 isolates and represents a quite divergent outgroup of the HIV-1 lineage. Our data suggest multiple introductions of HIV-1 in the human population and shed new light on the origin of the HIV-1 pandemic.
之前已对从加蓬捕获的黑猩猩身上分离出的两种猿猴免疫缺陷病毒（SIV）毒株（SIVCPZ - GAB和SIVCPZ - GAB2）进行了基因特征分析，结果表明它们在系统发育上与1型人类免疫缺陷病毒（HIV - 1）属于同一谱系。我们描述了从一只源自扎伊尔的野生捕获黑猩猩身上分离出的第三种与HIV - 1相关的病毒SIVCPZ - ANT的序列分析。这种病毒显示出与HIV - 1相同的基因结构，并且被发现与HIV - 1和SIVCPZ - GAB属于同一谱系。其结构蛋白与SIVCPZ - GAB的蛋白序列同一性在结构蛋白中从72%（Pol）到48%（Env）不等，同时发现一个特别不同的Vpu（与SIVCPZ - GAB只有25%的同一性）。与HIV - 1分离株中V3的高度差异形成对比的是，SIVCPZ分离株的V3区域异常保守。然而，SIVCPZ - ANT与SIVCPZ - GAB的序列相似程度并不比与HIV - 1分离株的更高，代表了HIV - 1谱系中一个相当不同的外类群。我们的数据表明HIV - 1在人类群体中有多次引入，并为HIV - 1大流行的起源提供了新的线索。
