Morison L, Buvé A, Zekeng L, Heyndrickx L, Anagonou S, Musonda R, Kahindo M, Weiss H A, Hayes R J, Laga M, Janssens W, van der Groen G
Tropical Epidemiology Group, London School of Hygiene and Tropical Medicine, UK.
AIDS. 2001 Aug;15 Suppl 4:S109-16. doi: 10.1097/00002030-200108004-00012.
To describe the distribution of HIV-1 subtypes in two cities with high HIV prevalence (Kisumu, Kenya and Ndola, Zambia) and two with relatively low prevalence (Cotonou, Benin and Yaoundé, Cameroon), and to examine whether the differences in prevalence of HIV infection could be due to the predominance within the infected populations of subtypes with differing efficiency of heterosexual transmission.
For around 100 randomly selected HIV-positive sera from the general population and 60 from sex workers in each city, the HIV-1 subtype was determined in the envfragment. For between 19 and 52 of the sera from the general population and 20-32 sera from sex workers, the subtype was also determined in the gag fragment.
Over 70% of infections in Cotonou, Yaoundé and Kisumu were with subtype A (by env). However, around one-half of subtype A infections in Cotonou and Yaoundé were found to be the circulating recombinant form CRF02_AG when the gag fragment was also examined. A large number of different HIV strains were found in Yaoundé, including some belonging to group O. Over 20% of infections in Kisumu and around 10% in Yaoundé were with isolated intersubtype recombinant forms. All but a few infections in Ndola were with subtype C and no recombinants were found.
The pattern of distribution of subtypes that we found does not suggest that differences in circulating subtypes play a major role in explaining the differences in prevalence of HIV-1 infection between the four cities. The emergence and spread of recombinants requires close surveillance to adapt testing strategies if needed, to inform vaccine development and to ascertain their role in the future spread of HIV.
描述在两个艾滋病病毒（HIV）高流行城市（肯尼亚的基苏木和赞比亚的恩多拉）以及两个相对低流行城市（贝宁的科托努和喀麦隆的雅温得）中HIV-1亚型的分布情况，并研究HIV感染率的差异是否可能归因于在感染人群中具有不同异性传播效率的亚型占主导地位。
在每个城市中，从普通人群中随机选取约100份HIV阳性血清，从性工作者中选取60份血清，在env片段中确定HIV-1亚型。对于普通人群中的19至52份血清以及性工作者中的20 - 32份血清，也在gag片段中确定亚型。
在科托努、雅温得和基苏木，超过70%的感染是由A型亚型引起的（通过env检测）。然而，当也检测gag片段时，发现在科托努和雅温得约一半的A型亚型感染是流行重组型CRF02_AG。在雅温得发现了大量不同的HIV毒株，包括一些属于O组的毒株。在基苏木超过20%的感染以及在雅温得约10%的感染是由孤立的亚型间重组型引起的。恩多拉除少数感染外均为C型亚型，未发现重组型。
我们发现的亚型分布模式并不表明流行亚型的差异在解释这四个城市间HIV-1感染率差异方面起主要作用。重组型的出现和传播需要密切监测，以便在需要时调整检测策略，为疫苗研发提供信息，并确定它们在HIV未来传播中的作用。
